Time course study of in situ expression of antigens following DNA-vaccination against VHS in rainbow trout (Oncorhynchus mykiss Walbaum) fry

Research output: Contribution to journalJournal article – Annual report year: 2005Researchpeer-review

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The present study was performed as a time course study of fish vaccinated with 20 mu g plasmid DNA vaccine encoding either the VHSV G-protein or the VHSV N-protein. Samples of the injection site were collected sequentially over a 7-week period. The study revealed an intense positive staining by immunohistochemistry for the viral G-protein mainly in the membrane of intact myocytes, most prominent by days 10-27, and with concomitant infiltration of inflammatory cells by days 13-38 that subsequently lead to a marked reduction in the number of myocytes expressing the G-protein. By immunofluorescence, infiltrating cells positive for MHC II, IgM, and C3 were demonstrated. By contrast, in fish vaccinated with the VHSV-N construct, fewer, diffusely positive myocytes were found, most prominent by days 13-38, these having a positive reaction for the N-protein mainly in the cytoplasm and variably in the membrane. N-protein positive myocytes did not attract infiltrating cells to the same degree. Positive reaction for the N-protein almost ceased by day 48 post-vaccination.
Original languageEnglish
JournalFish & shellfish immunology
Issue number1
Pages (from-to)27-41
Publication statusPublished - 2005
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • immunohistochemistry, DNA-vaccination, in situ expression, VHSV G-protein, inflammatory response, VHSV N-protein, rainbow trout
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ID: 3360893