Fluidic system for long-term in vitro culturing and monitoring of organotypic brain slices

Research output: Contribution to journalJournal article – Annual report year: 2015Researchpeer-review

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Brain slice preparations cultured in vitro have long been used as a simplified model for studying brain development, electrophysiology, neurodegeneration and neuroprotection. In this paper an open fluidic system developed for improved long term culturing of organotypic brain slices is presented. The positive effect of continuous flow of growth medium, and thus stability of the glucose concentration and waste removal, is simulated and compared to the effect of stagnant medium that is most often used in tissue culturing. Furthermore, placement of the tissue slices in the developed device was studied by numerical simulations in order to optimize the nutrient distribution. The device was tested by culturing transverse hippocampal slices from 7 days old NMRI mice for a duration of 14 days. The slices were inspected visually and the slices cultured in the fluidic system appeared to have preserved their structure better than the control slices cultured using the standard interface method.
Original languageEnglish
Article number71
JournalBiomedical Microdevices
Volume17
Issue number4
Number of pages7
ISSN1387-2176
DOIs
Publication statusPublished - 2015
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Fluidic, Hippocampus, Interface culturing, Tissue culturing, Brain, Electrophysiology, Fluidics, Brain development, Continuous flows, Glucose concentration, Hippocampal slice, Neurodegeneration, Nutrient distributions, Standard interface, Tissue, HASH(0x4f00450)
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