Schmallenberg virus experimental infection of sheep

Research output: Contribution to journalJournal article – Annual report year: 2013Researchpeer-review

  • Author: Wernike, Kerstin

    Friedrich-Loeffler-Institute, Germany

  • Author: Hoffmann, Bernd

    Friedrich-Loeffler-Institute, Germany

  • Author: Bréard, Emmanuel

    ANSES - French Agency for Food, Environmental and Occupational Health & Safety, France

  • Author: Bøtner, Anette

    Section for Virology, National Veterinary Institute, Technical University of Denmark, Lindholm, 4771, Kalvehave, Denmark

  • Author: Ponsart, Claire

    National Laboratory for Sanitary Controls in Breeding Animals, France

  • Author: Zientara, Stéphan

    ANSES - French Agency for Food, Environmental and Occupational Health & Safety, France

  • Author: Lohse, Louise

    Section for Virology, National Veterinary Institute, Technical University of Denmark, Lindholm, 4771, Kalvehave, Denmark

  • Author: Pozzi, Nathalie

    National Laboratory for Sanitary Controls in Breeding Animals, France

  • Author: Viarouge, Cyril

    ANSES - French Agency for Food, Environmental and Occupational Health & Safety, France

  • Author: Sarradin, Pierre

    Institut National de la Recherche Agronomique, France

  • Author: Leroux-Barc, Céline

    Institut National de la Recherche Agronomique, France

  • Author: Riou, Mickael

    Institut National de la Recherche Agronomique, France

  • Author: Laloy, Eve

    ANSES - French Agency for Food, Environmental and Occupational Health & Safety, France

  • Author: Breithaupt, Angele

    Friedrich-Loeffler-Institute, Germany

  • Author: Beer, Martin

    Friedrich-Loeffler-Institute, Germany

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Since late 2011, a novel orthobunyavirus, named Schmallenberg virus (SBV), has been implicated in many cases of severely malformed bovine and ovine offspring in Europe. In adult cattle, SBV is known to cause a mild transient disease; clinical signs include short febrile episodes, decreased milk production and diarrhoea for a few days. However, the knowledge about clinical signs and pathogenesis in adult sheep is limited.In the present study, adult sheep of European domestic breeds were inoculated with SBV either as cell culture grown virus or as virus with no history of passage in cell cultures. Various experimental set-ups were used. Sampling included blood collection at different time points during the experimental period and selected organ material at autopsy.Data from this study showed, that the RNAemic period in sheep was as short as reported for cattle; viral genome was detectable for about 3–5 days by real-time RT-PCR. In total, 13 out of 30 inoculated sheep became RNAemic, with the highest viral load in animals inoculated with virus from low cell culture passaged or the animal passaged material. Contact animals remained negative throughout the study. One RNAemic sheep showed diarrhoea for several days, but fever was not recorded in any of the animals. Antibodies were first detectable 10–14 days post inoculation. Viral RNA was detectable in spleen and lymph nodes up to day 44 post inoculation.In conclusion, as described for cattle, SBV-infection in adult sheep predominantly results in subclinical infection, transient RNAemia and a specific antibody response. Maintenance of viral RNA in the lymphoreticular system is observed for an extended period.
Original languageEnglish
JournalVeterinary Microbiology
Volume166
Issue number3-4
Pages (from-to)461-466
ISSN0378-1135
DOIs
Publication statusPublished - 2013
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Schmallenberg virus, Orthobunyavirus, Sheep, Experimental infection

ID: 58037286