Complete genome sequence of a multiple drug resistant Salmonella enterica serovar Typhi CT18

Research output: Contribution to journalJournal article – Annual report year: 2001Researchpeer-review

  • Author: Parkhill, J.

  • Author: Dougan, G.

  • Author: James, K.D.

  • Author: Thomson, N.R.

  • Author: Pickard, D.

  • Author: Wain, J.

  • Author: Churcher, C.

  • Author: Mungall, K.L.

  • Author: Bentley, S.D.

  • Author: Holden, M.T.G.

  • Author: Sebaihia, M.

  • Author: Baker, S.

  • Author: Basham, D.

  • Author: Brooks, K.

  • Author: Chillingworth, T.

  • Author: Connerton, P.

  • Author: Cronin, A.

  • Author: Davis, P.

  • Author: Davies, R.M.

  • Author: Dowd, L.

  • Author: White, N.

  • Author: Farrar, J.

  • Author: Feltwell, T.

  • Author: Hamlin, N.

  • Author: Haque, A.

  • Author: Hien, T.T.

  • Author: Holroyd, S.

  • Author: Jagels, K.

  • Author: Krogh, Anders Stærmose

    Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark

  • Author: Larsen, T. S.

  • Author: Leather, S.

  • Author: Moule, S.

  • Author: O'Gaora, P.

  • Author: Parry, C.

  • Author: Quail, M.

  • Author: Rutherford, K.

  • Author: Simmonds, M.

  • Author: Skelton, J.

  • Author: Stevens, K.

  • Author: Whitehead, S.

  • Author: Barrell, B.G.

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Salmonella enterica serovar Typhi (S. typhi) is the aetiological agent of typhoid fever, a serious invasive bacterial disease of humans with an annual global burden of approximately 16 million cases, leading to 600,000 fatalities(1). Many S. enterica serovars actively invade the mucosal surface of the intestine but are normally contained in healthy individuals by the local immune defence mechanisms. However, S. typhi has evolved the ability to spread to the deeper tissues of humans, including liver, spleen and bone marrow. Here we have sequenced the 4,809,037-base pair (bp) genome of a S. typhi (CT18) that is resistant to multiple drugs, revealing the presence of hundreds of insertions and deletions compared with the Escherichia coli genome, ranging in size from single genes to large islands. Notably, the genome sequence identifies over two hundred pseudogenes, several corresponding to genes that are known to contribute to virulence in Salmonella typhimurium. This genetic degradation may contribute to the human-restricted host range for S. typhi. CT18 harbours a 218,150-bp multiple-drug-resistance incH1 plasmid (pHCM1), and a 106,516-bp cryptic plasmid (pHCM2), which shows recent common ancestry with a virulence plasmid of Yersinia pestis.
Original languageEnglish
JournalNature
Volume413
Issue number6858
Pages (from-to)848-852
ISSN0028-0836
DOIs
Publication statusPublished - 2001
CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • cbs

ID: 3927063