Bioreactor process monitoring using an automated microfluidic platform for cell-based assays

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedings – Annual report year: 2016Researchpeer-review

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We report on a novel microfluidic system designed to monitor in real-time the concentration of live and dead cells in industrial cell production. Custom-made stepper motor actuated peristaltic pumps and valves, fluidic interconnections, sample-to-waste liquid management and image cytometry-based detection contribute to the high programmability and automation of this platform. Furthermore, this is to the best of our knowledge, the first use of Dean vortices to implement a wide range of dilution factors to highly concentrated cell samples. The combination of a curved channel geometry and high flow rates enables the rapid passive mixing and homogenization of the diluted cell plug.
Original languageEnglish
Title of host publication19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
Number of pages3
PublisherChemical and Biological Microsystems Society
Publication date2015
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
EventMicroTAS 2015: 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Gyeongju, Korea, Republic of
Duration: 25 Oct 201529 Oct 2015
Conference number: 19


ConferenceMicroTAS 2015
CountryKorea, Republic of

    Research areas

  • Programmable, Microfluidic system, Cell sample dilution, Mixing, Bioreactor

ID: 127331550