YGFP: a spectral variant of GFP

Research output: Contribution to journalJournal article – Annual report year: 2011Researchpeer-review

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We describe YGFP, a slow bleaching green fluorescent protein (GFP) with unique spectral properties. YGFP is derived from an Escherichia coli codon-optimized synthetic gfp, mutant 2 derivative. In addition to the GFP-mut 2 changes, it also carries S202F and T203I substitutions. YGFP can be used as a substitute for yellow fluorescent protein (YFP) in experiments in which two or more fluorescent proteins are fused to different cellular protein components, expanding the ability to study multiple labeled proteins in a cell at once.
Original languageEnglish
Issue number6
Pages (from-to)411-412
Publication statusPublished - 2011

Bibliographical note

Supplementary material for this article is available at www.BioTechniques.com/article/113691.

CitationsWeb of Science® Times Cited: No match on DOI

    Research areas

  • Fluorescent spectra, Fluorescent ParB foci, Photo-bleaching, Multifluorescence cell labeling, mOrange, Fluorescence microscopy

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