YGFP: a spectral variant of GFP

Flemming G. Hansen, Tove Atlung

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We describe YGFP, a slow bleaching green fluorescent protein (GFP) with unique spectral properties. YGFP is derived from an Escherichia coli codon-optimized synthetic gfp, mutant 2 derivative. In addition to the GFP-mut 2 changes, it also carries S202F and T203I substitutions. YGFP can be used as a substitute for yellow fluorescent protein (YFP) in experiments in which two or more fluorescent proteins are fused to different cellular protein components, expanding the ability to study multiple labeled proteins in a cell at once.
    Original languageEnglish
    JournalBioTechniques
    Volume50
    Issue number6
    Pages (from-to)411-412
    ISSN0736-6205
    DOIs
    Publication statusPublished - 2011

    Bibliographical note

    Supplementary material for this article is available at www.BioTechniques.com/article/113691.

    Keywords

    • Fluorescent spectra
    • Fluorescent ParB foci
    • Photo-bleaching
    • Multifluorescence cell labeling
    • mOrange
    • Fluorescence microscopy

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