YGFP: a spectral variant of GFP

Flemming G. Hansen; Tove Atlung

doi:10.2144/000113691

YGFP: a spectral variant of GFP

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Abstract

We describe YGFP, a slow bleaching green fluorescent protein (GFP) with unique spectral properties. YGFP is derived from an Escherichia coli codon-optimized synthetic gfp, mutant 2 derivative. In addition to the GFP-mut 2 changes, it also carries S202F and T203I substitutions. YGFP can be used as a substitute for yellow fluorescent protein (YFP) in experiments in which two or more fluorescent proteins are fused to different cellular protein components, expanding the ability to study multiple labeled proteins in a cell at once.

Original language	English
Journal	BioTechniques
Volume	50
Issue number	6
Pages (from-to)	411-412
ISSN	0736-6205
DOIs	https://doi.org/10.2144/000113691
Publication status	Published - 2011

Bibliographical note

Supplementary material for this article is available at www.BioTechniques.com/article/113691.

Keywords

Fluorescent spectra
Fluorescent ParB foci
Photo-bleaching
Multifluorescence cell labeling
mOrange
Fluorescence microscopy

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title = "YGFP: a spectral variant of GFP",

abstract = "We describe YGFP, a slow bleaching green fluorescent protein (GFP) with unique spectral properties. YGFP is derived from an Escherichia coli codon-optimized synthetic gfp, mutant 2 derivative. In addition to the GFP-mut 2 changes, it also carries S202F and T203I substitutions. YGFP can be used as a substitute for yellow fluorescent protein (YFP) in experiments in which two or more fluorescent proteins are fused to different cellular protein components, expanding the ability to study multiple labeled proteins in a cell at once.",

keywords = "Fluorescent spectra, Fluorescent ParB foci, Photo-bleaching, Multifluorescence cell labeling, mOrange, Fluorescence microscopy",

author = "Hansen, {Flemming G.} and Tove Atlung",

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AU - Hansen, Flemming G.

AU - Atlung, Tove

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N2 - We describe YGFP, a slow bleaching green fluorescent protein (GFP) with unique spectral properties. YGFP is derived from an Escherichia coli codon-optimized synthetic gfp, mutant 2 derivative. In addition to the GFP-mut 2 changes, it also carries S202F and T203I substitutions. YGFP can be used as a substitute for yellow fluorescent protein (YFP) in experiments in which two or more fluorescent proteins are fused to different cellular protein components, expanding the ability to study multiple labeled proteins in a cell at once.

AB - We describe YGFP, a slow bleaching green fluorescent protein (GFP) with unique spectral properties. YGFP is derived from an Escherichia coli codon-optimized synthetic gfp, mutant 2 derivative. In addition to the GFP-mut 2 changes, it also carries S202F and T203I substitutions. YGFP can be used as a substitute for yellow fluorescent protein (YFP) in experiments in which two or more fluorescent proteins are fused to different cellular protein components, expanding the ability to study multiple labeled proteins in a cell at once.

KW - Fluorescent spectra

KW - Fluorescent ParB foci

KW - Photo-bleaching

KW - Multifluorescence cell labeling

KW - mOrange

KW - Fluorescence microscopy

U2 - 10.2144/000113691

DO - 10.2144/000113691

M3 - Journal article

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VL - 50

SP - 411

EP - 412

JO - BioTechniques

JF - BioTechniques

SN - 0736-6205

IS - 6

ER -

YGFP: a spectral variant of GFP

Abstract

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Keywords

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