TY - JOUR
T1 - Xylose fermentation to biofuels (hydrogen and ethanol) by extreme thermophilic (70 C) mixed culture
AU - Chenxi, Zhao
AU - Karakashev, Dimitar Borisov
AU - Lu, W.
AU - Wang, H.
AU - Angelidaki, Irini
PY - 2010
Y1 - 2010
N2 - Combined biohydrogen and bioethanol (CHE) production from xylose was achieved by an extreme thermophilic (70 degrees C) mixed culture. Effect of initial pH, xylose, peptone, FeSO4, NaHCO3, yeast extract, trace mineral salts, vitamins, and phosphate buffer concentrations on bioethanol and biohydrogen yield was investigated in batch experiments. Results obtained showed that initial pH, concentration of xylose, peptone, and FeSO4 significantly affected biohydrogen and bioethanol production, while the concentration of NaHCO3 was only significant for bioethanol production. By changing cultivation conditions the culture could be directed to mainly produce ethanol with maximum ethanol yield of 1.60 mol ethanol/mol-xylose corresponding to 95.8% of the theoretical ethanol yield based on degradation of xylose through ethanologenic pathway, or mainly hydrogen with maximum hydrogen yield of 1.84 mol H-2/mol-xylose corresponding to 55% of the theoretical hydrogen yield based on acetate metabolic pathway. An empirical model was established to reveal the quantitative effect of factors significant for biohydrogen (quadratic model) production and for bioethanol (linear model) production. Changes in hydrogen/ethanol yields observed were due to the shift of the metabolic pathway between ethanol or hydrogen production, rather than changes in bacterial community composition at genus level. Thermoanaerobacter related bacteria were found to be the dominant hydrogen/ethanol producers. (C) 2010 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.
AB - Combined biohydrogen and bioethanol (CHE) production from xylose was achieved by an extreme thermophilic (70 degrees C) mixed culture. Effect of initial pH, xylose, peptone, FeSO4, NaHCO3, yeast extract, trace mineral salts, vitamins, and phosphate buffer concentrations on bioethanol and biohydrogen yield was investigated in batch experiments. Results obtained showed that initial pH, concentration of xylose, peptone, and FeSO4 significantly affected biohydrogen and bioethanol production, while the concentration of NaHCO3 was only significant for bioethanol production. By changing cultivation conditions the culture could be directed to mainly produce ethanol with maximum ethanol yield of 1.60 mol ethanol/mol-xylose corresponding to 95.8% of the theoretical ethanol yield based on degradation of xylose through ethanologenic pathway, or mainly hydrogen with maximum hydrogen yield of 1.84 mol H-2/mol-xylose corresponding to 55% of the theoretical hydrogen yield based on acetate metabolic pathway. An empirical model was established to reveal the quantitative effect of factors significant for biohydrogen (quadratic model) production and for bioethanol (linear model) production. Changes in hydrogen/ethanol yields observed were due to the shift of the metabolic pathway between ethanol or hydrogen production, rather than changes in bacterial community composition at genus level. Thermoanaerobacter related bacteria were found to be the dominant hydrogen/ethanol producers. (C) 2010 Professor T. Nejat Veziroglu. Published by Elsevier Ltd. All rights reserved.
U2 - 10.1016/j.ijhydene.2010.01.082
DO - 10.1016/j.ijhydene.2010.01.082
M3 - Journal article
SN - 0360-3199
VL - 35
SP - 3415
EP - 3422
JO - International Journal of Hydrogen Energy
JF - International Journal of Hydrogen Energy
IS - 8
ER -
