Wiring cell growth to product formation

Josi Buerger, Luisa S Gronenberg, Hans Jasper Genee, Morten Otto Alexander Sommer*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Microbial cell factories offer new and sustainable production routes for high-value chemicals. However, identification of high producers within a library of clones remains a challenge. When product formation is coupled to growth, millions of metabolic variants can be effectively interrogated by growth selection, dramatically increasing the throughput of strain evaluation. While growth-coupled selections for cell factories have a long history of success based on metabolite auxotrophies and toxic antimetabolites, such methods are generally restricted to molecules native to their host metabolism. New synthetic biology tools offer the opportunity to rewire cellular metabolism to depend on specific and non-native products for growth.
Original languageEnglish
JournalCurrent Opinion in Biotechnology
Volume59
Pages (from-to)85-92
ISSN0958-1669
DOIs
Publication statusPublished - 2019

Keywords

  • Biology
  • Physiology
  • Synthetic biology
  • Cellular metabolism
  • Growth selection
  • High producers
  • High-value chemicals
  • Microbial cell factories
  • Product formation
  • Strain evaluation
  • Sustainable production
  • Metabolism

Cite this

Buerger, Josi ; Gronenberg, Luisa S ; Genee, Hans Jasper ; Sommer, Morten Otto Alexander. / Wiring cell growth to product formation. In: Current Opinion in Biotechnology. 2019 ; Vol. 59. pp. 85-92.
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abstract = "Microbial cell factories offer new and sustainable production routes for high-value chemicals. However, identification of high producers within a library of clones remains a challenge. When product formation is coupled to growth, millions of metabolic variants can be effectively interrogated by growth selection, dramatically increasing the throughput of strain evaluation. While growth-coupled selections for cell factories have a long history of success based on metabolite auxotrophies and toxic antimetabolites, such methods are generally restricted to molecules native to their host metabolism. New synthetic biology tools offer the opportunity to rewire cellular metabolism to depend on specific and non-native products for growth.",
keywords = "Biology, Physiology, Synthetic biology, Cellular metabolism, Growth selection, High producers, High-value chemicals, Microbial cell factories, Product formation, Strain evaluation, Sustainable production, Metabolism",
author = "Josi Buerger and Gronenberg, {Luisa S} and Genee, {Hans Jasper} and Sommer, {Morten Otto Alexander}",
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Wiring cell growth to product formation. / Buerger, Josi; Gronenberg, Luisa S; Genee, Hans Jasper; Sommer, Morten Otto Alexander.

In: Current Opinion in Biotechnology, Vol. 59, 2019, p. 85-92.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

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AU - Buerger, Josi

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AU - Genee, Hans Jasper

AU - Sommer, Morten Otto Alexander

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N2 - Microbial cell factories offer new and sustainable production routes for high-value chemicals. However, identification of high producers within a library of clones remains a challenge. When product formation is coupled to growth, millions of metabolic variants can be effectively interrogated by growth selection, dramatically increasing the throughput of strain evaluation. While growth-coupled selections for cell factories have a long history of success based on metabolite auxotrophies and toxic antimetabolites, such methods are generally restricted to molecules native to their host metabolism. New synthetic biology tools offer the opportunity to rewire cellular metabolism to depend on specific and non-native products for growth.

AB - Microbial cell factories offer new and sustainable production routes for high-value chemicals. However, identification of high producers within a library of clones remains a challenge. When product formation is coupled to growth, millions of metabolic variants can be effectively interrogated by growth selection, dramatically increasing the throughput of strain evaluation. While growth-coupled selections for cell factories have a long history of success based on metabolite auxotrophies and toxic antimetabolites, such methods are generally restricted to molecules native to their host metabolism. New synthetic biology tools offer the opportunity to rewire cellular metabolism to depend on specific and non-native products for growth.

KW - Biology

KW - Physiology

KW - Synthetic biology

KW - Cellular metabolism

KW - Growth selection

KW - High producers

KW - High-value chemicals

KW - Microbial cell factories

KW - Product formation

KW - Strain evaluation

KW - Sustainable production

KW - Metabolism

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