Widespread Occurrence of the Mycotoxin Fumonisin B-2 in Wine

Jesper Mølgaard Mogensen, Thomas Ostenfeld Larsen, Kristian Fog Nielsen

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    Fumonisins are important mycotoxins because they are suspected to cause human and animal toxicoses by the consumption of contaminated corn-based food and feeds. However, with the discovery of fumonisin production in grapes by Aspergillus niger, wine may also be a fumonisin-containing commodity. In the present study, we have developed a simple and quantitative cation-exchange-based purification method for the subsequent isotope dilution liquid chromatography tandem mass spectrometry (LC-MS/MS) determination of fumonisins in wine. A comparative study of seven different solid-phase extraction (SPE) columns showed that polymeric mixed-mode reversed-phase (RP) cation-exchange columns were superior to classic silica-based cation and mixed-mode cation-exchange columns. A total of 77 wine samples from 13 countries were subsequently tested, and surprisingly, 18 (23%) were found to contain fumonisin B-2 in the range of 1-25 mu g/L. These findings were further confirmed by immunoaffinity purification and re-analysis of the positive cation-exchanged extracts.
    Original languageEnglish
    JournalJournal of Agricultural and Food Chemistry
    Issue number8
    Pages (from-to)4853-4857
    Publication statusPublished - 2010


    • wine
    • grapes
    • Fumonisin
    • Aspergillus niger

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