Whole-blood culture is a valid low-cost method to measure monocytic cytokines - A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes

Camilla T. Damsgaard; Lotte Lauritzen; Philip C. Calder; Tanja Kjær; Hanne Frøkiær

doi:10.1016/j.jim.2008.10.005

Whole-blood culture is a valid low-cost method to measure monocytic cytokines - A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes

Camilla T. Damsgaard, Lotte Lauritzen, Philip C. Calder, Tanja Kjær, Hanne Frøkiær

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Whole-blood and peripheral blood mononuclear cell (PBMC) cultures are used as non-validated surrogate measures of monocytic cytokine production. The aim of this investigation was to compare ex vivo cytokine production from human whole-blood and PBMC with that from isolated monocytes. We also assessed the intra- and inter-individual variation in cytokine production. In 64 healthy men (age 19-40 years) IL-6, TNF and IL-10 were measured by enzyme-linked immunosorbent assay in supernatants from whole-blood, PBMC and monocytes cultured 24 h with lipopolysaccharide (LPS) or UV-killed L acidophilus. Cytokines produced from whole-blood was found to be more strongly correlated with monocytic cytokines than cytokines from PBMC, particularly after LPS-stimulation: r=0.57, P= 50% smaller than the inter-individual variation (P

Original language	English
Journal	Journal of Immunological Methods
Volume	340
Issue number	2
Pages (from-to)	95-101
ISSN	0022-1759
DOIs	https://doi.org/10.1016/j.jim.2008.10.005
Publication status	Published - 2009

Keywords

Mononuclear cell
Inflammatory cytokines
Whole-blood culture
Monocyte

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10.1016/j.jim.2008.10.005

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title = "Whole-blood culture is a valid low-cost method to measure monocytic cytokines - A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes",

abstract = "Whole-blood and peripheral blood mononuclear cell (PBMC) cultures are used as non-validated surrogate measures of monocytic cytokine production. The aim of this investigation was to compare ex vivo cytokine production from human whole-blood and PBMC with that from isolated monocytes. We also assessed the intra- and inter-individual variation in cytokine production. In 64 healthy men (age 19-40 years) IL-6, TNF and IL-10 were measured by enzyme-linked immunosorbent assay in supernatants from whole-blood, PBMC and monocytes cultured 24 h with lipopolysaccharide (LPS) or UV-killed L acidophilus. Cytokines produced from whole-blood was found to be more strongly correlated with monocytic cytokines than cytokines from PBMC, particularly after LPS-stimulation: r=0.57, P= 50% smaller than the inter-individual variation (P",

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author = "Damsgaard, {Camilla T.} and Lotte Lauritzen and Calder, {Philip C.} and Tanja Kj{\ae}r and Hanne Fr{\o}ki{\ae}r",

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language = "English",

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Whole-blood culture is a valid low-cost method to measure monocytic cytokines - A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes. / Damsgaard, Camilla T.; Lauritzen, Lotte; Calder, Philip C. et al.
In: Journal of Immunological Methods, Vol. 340, No. 2, 2009, p. 95-101.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Whole-blood culture is a valid low-cost method to measure monocytic cytokines - A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes

AU - Damsgaard, Camilla T.

AU - Lauritzen, Lotte

AU - Calder, Philip C.

AU - Kjær, Tanja

AU - Frøkiær, Hanne

PY - 2009

Y1 - 2009

N2 - Whole-blood and peripheral blood mononuclear cell (PBMC) cultures are used as non-validated surrogate measures of monocytic cytokine production. The aim of this investigation was to compare ex vivo cytokine production from human whole-blood and PBMC with that from isolated monocytes. We also assessed the intra- and inter-individual variation in cytokine production. In 64 healthy men (age 19-40 years) IL-6, TNF and IL-10 were measured by enzyme-linked immunosorbent assay in supernatants from whole-blood, PBMC and monocytes cultured 24 h with lipopolysaccharide (LPS) or UV-killed L acidophilus. Cytokines produced from whole-blood was found to be more strongly correlated with monocytic cytokines than cytokines from PBMC, particularly after LPS-stimulation: r=0.57, P= 50% smaller than the inter-individual variation (P

AB - Whole-blood and peripheral blood mononuclear cell (PBMC) cultures are used as non-validated surrogate measures of monocytic cytokine production. The aim of this investigation was to compare ex vivo cytokine production from human whole-blood and PBMC with that from isolated monocytes. We also assessed the intra- and inter-individual variation in cytokine production. In 64 healthy men (age 19-40 years) IL-6, TNF and IL-10 were measured by enzyme-linked immunosorbent assay in supernatants from whole-blood, PBMC and monocytes cultured 24 h with lipopolysaccharide (LPS) or UV-killed L acidophilus. Cytokines produced from whole-blood was found to be more strongly correlated with monocytic cytokines than cytokines from PBMC, particularly after LPS-stimulation: r=0.57, P= 50% smaller than the inter-individual variation (P

KW - Mononuclear cell

KW - Inflammatory cytokines

KW - Whole-blood culture

KW - Monocyte

U2 - 10.1016/j.jim.2008.10.005

DO - 10.1016/j.jim.2008.10.005

M3 - Journal article

SN - 0022-1759

VL - 340

SP - 95

EP - 101

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 2

ER -

Whole-blood culture is a valid low-cost method to measure monocytic cytokines - A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes

Abstract

Keywords

UN SDGs

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