Whole-blood culture is a valid low-cost method to measure monocytic cytokines - A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes

Camilla T. Damsgaard, Lotte Lauritzen, Philip C. Calder, Tanja Kjær, Hanne Frøkiær

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Whole-blood and peripheral blood mononuclear cell (PBMC) cultures are used as non-validated surrogate measures of monocytic cytokine production. The aim of this investigation was to compare ex vivo cytokine production from human whole-blood and PBMC with that from isolated monocytes. We also assessed the intra- and inter-individual variation in cytokine production. In 64 healthy men (age 19-40 years) IL-6, TNF and IL-10 were measured by enzyme-linked immunosorbent assay in supernatants from whole-blood, PBMC and monocytes cultured 24 h with lipopolysaccharide (LPS) or UV-killed L acidophilus. Cytokines produced from whole-blood was found to be more strongly correlated with monocytic cytokines than cytokines from PBMC, particularly after LPS-stimulation: r=0.57, P= 50% smaller than the inter-individual variation (P
    Original languageEnglish
    JournalJournal of Immunological Methods
    Volume340
    Issue number2
    Pages (from-to)95-101
    ISSN0022-1759
    DOIs
    Publication statusPublished - 2009

    Keywords

    • Mononuclear cell
    • Inflammatory cytokines
    • Whole-blood culture
    • Monocyte

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