Whole-blood culture is a valid low-cost method to measure monocytic cytokines - A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes

Camilla T. Damsgaard, Lotte Lauritzen, Philip C. Calder, Tanja Kjær, Hanne Frøkiær

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Whole-blood and peripheral blood mononuclear cell (PBMC) cultures are used as non-validated surrogate measures of monocytic cytokine production. The aim of this investigation was to compare ex vivo cytokine production from human whole-blood and PBMC with that from isolated monocytes. We also assessed the intra- and inter-individual variation in cytokine production. In 64 healthy men (age 19-40 years) IL-6, TNF and IL-10 were measured by enzyme-linked immunosorbent assay in supernatants from whole-blood, PBMC and monocytes cultured 24 h with lipopolysaccharide (LPS) or UV-killed L acidophilus. Cytokines produced from whole-blood was found to be more strongly correlated with monocytic cytokines than cytokines from PBMC, particularly after LPS-stimulation: r=0.57, P= 50% smaller than the inter-individual variation (P
    Original languageEnglish
    JournalJournal of Immunological Methods
    Volume340
    Issue number2
    Pages (from-to)95-101
    ISSN0022-1759
    DOIs
    Publication statusPublished - 2009

    Keywords

    • Mononuclear cell
    • Inflammatory cytokines
    • Whole-blood culture
    • Monocyte

    Cite this

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    title = "Whole-blood culture is a valid low-cost method to measure monocytic cytokines - A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes",
    abstract = "Whole-blood and peripheral blood mononuclear cell (PBMC) cultures are used as non-validated surrogate measures of monocytic cytokine production. The aim of this investigation was to compare ex vivo cytokine production from human whole-blood and PBMC with that from isolated monocytes. We also assessed the intra- and inter-individual variation in cytokine production. In 64 healthy men (age 19-40 years) IL-6, TNF and IL-10 were measured by enzyme-linked immunosorbent assay in supernatants from whole-blood, PBMC and monocytes cultured 24 h with lipopolysaccharide (LPS) or UV-killed L acidophilus. Cytokines produced from whole-blood was found to be more strongly correlated with monocytic cytokines than cytokines from PBMC, particularly after LPS-stimulation: r=0.57, P= 50{\%} smaller than the inter-individual variation (P",
    keywords = "Mononuclear cell, Inflammatory cytokines, Whole-blood culture, Monocyte",
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    year = "2009",
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    volume = "340",
    pages = "95--101",
    journal = "Journal of Immunological Methods",
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    Whole-blood culture is a valid low-cost method to measure monocytic cytokines - A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes. / Damsgaard, Camilla T.; Lauritzen, Lotte; Calder, Philip C.; Kjær, Tanja; Frøkiær, Hanne.

    In: Journal of Immunological Methods, Vol. 340, No. 2, 2009, p. 95-101.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Whole-blood culture is a valid low-cost method to measure monocytic cytokines - A comparison of cytokine production in cultures of human whole-blood, mononuclear cells and monocytes

    AU - Damsgaard, Camilla T.

    AU - Lauritzen, Lotte

    AU - Calder, Philip C.

    AU - Kjær, Tanja

    AU - Frøkiær, Hanne

    PY - 2009

    Y1 - 2009

    N2 - Whole-blood and peripheral blood mononuclear cell (PBMC) cultures are used as non-validated surrogate measures of monocytic cytokine production. The aim of this investigation was to compare ex vivo cytokine production from human whole-blood and PBMC with that from isolated monocytes. We also assessed the intra- and inter-individual variation in cytokine production. In 64 healthy men (age 19-40 years) IL-6, TNF and IL-10 were measured by enzyme-linked immunosorbent assay in supernatants from whole-blood, PBMC and monocytes cultured 24 h with lipopolysaccharide (LPS) or UV-killed L acidophilus. Cytokines produced from whole-blood was found to be more strongly correlated with monocytic cytokines than cytokines from PBMC, particularly after LPS-stimulation: r=0.57, P= 50% smaller than the inter-individual variation (P

    AB - Whole-blood and peripheral blood mononuclear cell (PBMC) cultures are used as non-validated surrogate measures of monocytic cytokine production. The aim of this investigation was to compare ex vivo cytokine production from human whole-blood and PBMC with that from isolated monocytes. We also assessed the intra- and inter-individual variation in cytokine production. In 64 healthy men (age 19-40 years) IL-6, TNF and IL-10 were measured by enzyme-linked immunosorbent assay in supernatants from whole-blood, PBMC and monocytes cultured 24 h with lipopolysaccharide (LPS) or UV-killed L acidophilus. Cytokines produced from whole-blood was found to be more strongly correlated with monocytic cytokines than cytokines from PBMC, particularly after LPS-stimulation: r=0.57, P= 50% smaller than the inter-individual variation (P

    KW - Mononuclear cell

    KW - Inflammatory cytokines

    KW - Whole-blood culture

    KW - Monocyte

    U2 - 10.1016/j.jim.2008.10.005

    DO - 10.1016/j.jim.2008.10.005

    M3 - Journal article

    VL - 340

    SP - 95

    EP - 101

    JO - Journal of Immunological Methods

    JF - Journal of Immunological Methods

    SN - 0022-1759

    IS - 2

    ER -