Whey-reduced weight gain is associated with a temporary growth reduction in young mice fed a high-fat diet

Britt Tranberg, Andreas N. Madsen, Axel K. Hansen, Lars Hellgren

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Whey protein consumption reportedly alleviates parameters of the metabolic syndrome. Here, we investigated the effects of whey protein isolate (whey) in
    young mice fed a high-fat diet. We hypothesized that whey as the sole protein source reduced early weight gain associated with retarded growth and decreased
    concentration of insulin-like growth factor-1. Moreover, we hypothesized that these changes were explained by increased nitrogen loss via elevated urea
    production and/or increased energy expenditure. Male 5-week-old C57BL/6 mice were fed high-fat diets with the protein source being either whey, casein or a
    combination of both for 5 weeks. After 1, 3 or 5 weeks, respectively, the mice were subjected to a meal challenge with measurements of blood and urinary urea
    before and 1 and 3 h after eating a weighed meal of their respective diets. In a subset of mice, energy expenditure was measured by indirect calorimetry during
    the first week of dietary intervention. Observed exclusively during the first week of intervention, whey significantly reduced body length (Pb.01) and weight gain
    (Pb.001) correlating positively with plasma concentrations of insulin-like growth factor-1. The combination diet displayed intermediate results indicating an
    interactive effect. Urea production, urea cycle activity, food intake and energy expenditure were unaffected by protein source. In conclusion, whey decreased
    growth-related parameters exclusively during the first week of dietary intervention. The early effect of whey could not be explained by food intake, energy expenditure, urea production or urea cycle activity but was correlated with plasma levels of insulin-like growth factor-1.
    Original languageEnglish
    JournalJournal of Nutritional Biochemistry
    Volume26
    Issue number1
    Pages (from-to)9–15
    Number of pages7
    ISSN0955-2863
    DOIs
    Publication statusPublished - 2015

    Keywords

    • Whey
    • Mice
    • High-fat diet
    • Insulin-like growth factor-1
    • Urea

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