WEFTA interlaboratory comparison on total lipid determination in fishery products using the Smedes method

Karl Horst, J. Oehlenschaeger, K. Bakaert, J. P. Berge, A. Cadun, G. Duflos, B. M. Poli, M. Tejada, S. Testi, Maike Timm Heinrich

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Lipid determination by the Smedes method was tested in an interlaboratory trial performed by 9 laboratories from 7 countries belonging to the West European Fish Technologists Association Analytical Methods Working Group. 5 samples of fish and fishery products with different lipid contents, including 2 blind duplicates, were distributed among the participants. All laboratories applied a slightly modified Smedes method, which included extraction of lipids by cyclohexane and isopropanol, transfer of lipids to the cyclohexane phase by addition of water, phase separation by centrifugation and gravimetric lipid determination. The relative s.d. for reproducibility (RSDR) was between 4.11 and 6.31% for samples with moderate (7%) and high (14%) lipid contents, depending on the sample. Larger SDs among the laboratories were obtained for a cod sample with low lipid content of 0.5%. The method is judged to be suitable as a routine method for lipid determination in fish and fishery products.
Original languageEnglish
JournalA O A C International. Journal
Volume95
Issue number2
Pages (from-to)489-493
ISSN1060-3271
DOIs
Publication statusPublished - 2012

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