Vitamin D vitamers affect vitamin D status differently in young healthy males

Jette Jakobsen*, Elisabeth Anne Wreford Andersen, Tue Christensen, Rikke Andersen, Susanne Bügel

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Dietary intake of vitamin D includes vitamin D3 (vitD3), 25-hydroxyvitamin D3 (25OH-D3), and vitamin D2 (vitD2). However, the bioactivity of the different species has not been scientifically established. The hypothesis in this study was that vitD3, 25OH-D3, and vitD2 have an equal effect on 25-hydroxyvitamin D in serum (vitamin D status). To test our hypothesis, we performed a randomized, crossover study. Twelve young males consumed 10 µg/day vitD3 during a four-week run-in period, followed by 3 × 6 weeks of 10 µg/day vitD3, 10 µg/day 25OH-D3, and 10 µg/day vitD2. The content of vitD3, vitD2, 25OH-D3, and 25-hydroxyvitamin D2 (25OH-D2) in serum was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The hypothesis that the three sources of vitamin D affect vitamin D status equally was rejected. Based on the assumption that 1 µg vitD3/day will show an increase in vitamin D status of 1.96 nmol/L, the results showed that 23 µg vitD2 and 6.8 µg 25OH-D3 was similar to 10 µg vitD3. These results demonstrate that further investigations are necessary to determine how to quantify the total vitamin D activity based on chemical quantification of the individual vitamin D metabolites to replace the total vitamin D activity assessed in biological rat models.

Original languageEnglish
JournalNutrients
Volume10
Issue number1
Number of pages12
ISSN2072-6643
DOIs
Publication statusPublished - 2018

Keywords

  • 25-hydroxyvitamin D3
  • Bioactivity
  • Humans
  • Supplements
  • Vitamin D2
  • Vitamin D3

Cite this

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title = "Vitamin D vitamers affect vitamin D status differently in young healthy males",
abstract = "Dietary intake of vitamin D includes vitamin D3 (vitD3), 25-hydroxyvitamin D3 (25OH-D3), and vitamin D2 (vitD2). However, the bioactivity of the different species has not been scientifically established. The hypothesis in this study was that vitD3, 25OH-D3, and vitD2 have an equal effect on 25-hydroxyvitamin D in serum (vitamin D status). To test our hypothesis, we performed a randomized, crossover study. Twelve young males consumed 10 µg/day vitD3 during a four-week run-in period, followed by 3 × 6 weeks of 10 µg/day vitD3, 10 µg/day 25OH-D3, and 10 µg/day vitD2. The content of vitD3, vitD2, 25OH-D3, and 25-hydroxyvitamin D2 (25OH-D2) in serum was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The hypothesis that the three sources of vitamin D affect vitamin D status equally was rejected. Based on the assumption that 1 µg vitD3/day will show an increase in vitamin D status of 1.96 nmol/L, the results showed that 23 µg vitD2 and 6.8 µg 25OH-D3 was similar to 10 µg vitD3. These results demonstrate that further investigations are necessary to determine how to quantify the total vitamin D activity based on chemical quantification of the individual vitamin D metabolites to replace the total vitamin D activity assessed in biological rat models.",
keywords = "25-hydroxyvitamin D3, Bioactivity, Humans, Supplements, Vitamin D2, Vitamin D3",
author = "Jette Jakobsen and {Wreford Andersen}, {Elisabeth Anne} and Tue Christensen and Rikke Andersen and Susanne B{\"u}gel",
year = "2018",
doi = "10.3390/nu10010012",
language = "English",
volume = "10",
journal = "Nutrients",
issn = "2072-6643",
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}

Vitamin D vitamers affect vitamin D status differently in young healthy males. / Jakobsen, Jette; Wreford Andersen, Elisabeth Anne; Christensen, Tue; Andersen, Rikke; Bügel, Susanne.

In: Nutrients, Vol. 10, No. 1, 2018.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Vitamin D vitamers affect vitamin D status differently in young healthy males

AU - Jakobsen, Jette

AU - Wreford Andersen, Elisabeth Anne

AU - Christensen, Tue

AU - Andersen, Rikke

AU - Bügel, Susanne

PY - 2018

Y1 - 2018

N2 - Dietary intake of vitamin D includes vitamin D3 (vitD3), 25-hydroxyvitamin D3 (25OH-D3), and vitamin D2 (vitD2). However, the bioactivity of the different species has not been scientifically established. The hypothesis in this study was that vitD3, 25OH-D3, and vitD2 have an equal effect on 25-hydroxyvitamin D in serum (vitamin D status). To test our hypothesis, we performed a randomized, crossover study. Twelve young males consumed 10 µg/day vitD3 during a four-week run-in period, followed by 3 × 6 weeks of 10 µg/day vitD3, 10 µg/day 25OH-D3, and 10 µg/day vitD2. The content of vitD3, vitD2, 25OH-D3, and 25-hydroxyvitamin D2 (25OH-D2) in serum was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The hypothesis that the three sources of vitamin D affect vitamin D status equally was rejected. Based on the assumption that 1 µg vitD3/day will show an increase in vitamin D status of 1.96 nmol/L, the results showed that 23 µg vitD2 and 6.8 µg 25OH-D3 was similar to 10 µg vitD3. These results demonstrate that further investigations are necessary to determine how to quantify the total vitamin D activity based on chemical quantification of the individual vitamin D metabolites to replace the total vitamin D activity assessed in biological rat models.

AB - Dietary intake of vitamin D includes vitamin D3 (vitD3), 25-hydroxyvitamin D3 (25OH-D3), and vitamin D2 (vitD2). However, the bioactivity of the different species has not been scientifically established. The hypothesis in this study was that vitD3, 25OH-D3, and vitD2 have an equal effect on 25-hydroxyvitamin D in serum (vitamin D status). To test our hypothesis, we performed a randomized, crossover study. Twelve young males consumed 10 µg/day vitD3 during a four-week run-in period, followed by 3 × 6 weeks of 10 µg/day vitD3, 10 µg/day 25OH-D3, and 10 µg/day vitD2. The content of vitD3, vitD2, 25OH-D3, and 25-hydroxyvitamin D2 (25OH-D2) in serum was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The hypothesis that the three sources of vitamin D affect vitamin D status equally was rejected. Based on the assumption that 1 µg vitD3/day will show an increase in vitamin D status of 1.96 nmol/L, the results showed that 23 µg vitD2 and 6.8 µg 25OH-D3 was similar to 10 µg vitD3. These results demonstrate that further investigations are necessary to determine how to quantify the total vitamin D activity based on chemical quantification of the individual vitamin D metabolites to replace the total vitamin D activity assessed in biological rat models.

KW - 25-hydroxyvitamin D3

KW - Bioactivity

KW - Humans

KW - Supplements

KW - Vitamin D2

KW - Vitamin D3

U2 - 10.3390/nu10010012

DO - 10.3390/nu10010012

M3 - Journal article

C2 - 29295513

AN - SCOPUS:85039442587

VL - 10

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 1

ER -