Virulence, immunogenicity and vaccine properties of a novel chimeric pestivirus

Thomas Bruun Rasmussen, Åse Uttenthal, Ilona Reimann, Jens Nielsen, Klaus Depner, Martin Beer

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A chimeric pestivirus of border disease virus Gifhorn and bovine viral diarrhea virus CP7 (Meyers et al., 1996) was constructed. Virulence, immunogenicity and vaccine properties of the chimeric virus were studied in a vaccination–challenge experiment in pigs. The chimeric virus proved to be avirulent and neither chimeric virus nor viral RNA was detected in serum after vaccination. The safety of the vaccine was tested by horizontal transmission to sentinel pigs, which remained uninfected. The vaccine efficacy was examined by challenge infection with classical swine fever virus (CSFV) Eystrup. In ‘challenge controls’, the viral load of CSFV coincided with the development of pronounced clinical symptoms. In contrast, the vaccinated pigs showed transient and weak clinical signs. Analysis of the viral load in these pigs showed 1000-fold lower viral RNA levels compared to ‘challenge controls' and horizontal transmission of challenge virus to sentinel pigs was not observed. A supplementary figure is available in JGV Online
    Original languageEnglish
    JournalJournal of General Virology
    Volume88
    Issue number2
    Pages (from-to)481-486
    ISSN0022-1317
    DOIs
    Publication statusPublished - 2007

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