Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are difficult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.
|Title of host publication||Plant Gene Silencing : Methods and Protocols|
|Publication status||Published - 2015|
|Series||Methods in Molecular Biology|
- Arbuscular mycorrhizal symbiosis
- Legume–Rhizobium symbiosis