VIGS for dissecting mechanisms involved in the symbiotic interaction of microbes with plants

Mette Grønlund

Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

Abstract

Virus-induced gene silencing (VIGS) is an alternative reverse genetics tool for silencing of genes in some plants which are difficult to transform. The pea early browning virus (PEBV) has been developed as a VIGS vector and used in pea for functional analysis of several genes. Here, a PEBV-VIGS protocol is described which is suitable for reverse genetics studies in pea for genes involved in the symbiosis with arbuscular mycorrhizal fungi and Rhizobium.
Original languageEnglish
Title of host publicationPlant Gene Silencing : Methods and Protocols
Publication date2015
Pages255-265
ISBN (Print)978-1-4939-2452-3
ISBN (Electronic)978-1-4939-2453-0
DOIs
Publication statusPublished - 2015
SeriesMethods in Molecular Biology
Volume1287
ISSN1064-3745

Keywords

  • VIGS
  • PEBV
  • Arbuscular mycorrhizal symbiosis
  • Legume–Rhizobium symbiosis

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