Vibrio anguillarum serogroup O3 and V. anguillarum-like serogroup O3 cross-reactive species - comparison and characterization

T. Tiainen, Karl Pedersen, J.L. Larsen

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Abstract

Forty-five Vibrio anguillarum-like isolates reacting with V. anguillarum serogroup 0 3 antiserum were examined in 30 characters to clarify their phenotypical properties, while their genotype was examined by ribotyping. The strains were isolated from diseased and dead fish or from environmental sources such as
water, sediment, plankton, and faeces and gills of healthy fish. Phenotypically, the
similarity of all the strains was more than 90%. However, significant differences
between the fish-associated and environmental strains were detected. Biochemically, deviations were found in the Voges-Proskauer test and lysine decarboxylase reaction. Clustering analysis of the ribotypes showed two distinct clusters with a similarity of only 32%. Two strains representing each of these groups were used in a LD,,study, which showed some difference also in the pathogenicity between environmental and fish strains. It is suggested that the environmental strains belong to another species than V. anguillarum, but serologically cross-reacting with the I/. anguillarum serogroup 03.
The ribotyping as u7ell as biochemical results indicated that the environmental strains possibly belong to Vibrio aestuarianus. The bona3de l? anguillarum serogroup 0 3 strains proved to be very homogeneous both phenotypically and genotypically, and the similarity of ribotypes was more than 96%. The V. anguillarum-like, serogroup 03-reactive strains from the environment were more heterogeneous in their biochemical behaviour, and showed an approximately 70% similarity in ribotypes.
Original languageEnglish
JournalJournal of Applied Microbiology
Volume82
Pages (from-to)211-218
ISSN1364-5072
Publication statusPublished - 1997
Externally publishedYes

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