Viability and proliferation of rat MSCs on adhesion protein-modified PET and PU scaffolds

Ylva Gustafsson, Johannes Haag, Philipp Jungebluth, Vanessa Lundin, Mei Ling Lim, Silvia Baiguera, Fatemeh Ajalloueian, Costantino Del Gaudio, Alessandra Bianco, Guido Moll, Sebastian Sjoqvist, Greg Lemon, Ana Isabel Teixeira, Paolo Macchiarini

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In 2011, the first in-man successful transplantation of a tissue engineered trachea-bronchial graft, using a synthetic POSS-PCU nanocomposite construct seeded with autologous stem cells, was performed. To further improve this technology, we investigated the feasibility of using polymers with a three dimensional structure more closely mimicking the morphology and size scale of native extracellular matrix (ECM) fibers. We therefore investigated the in vitro biocompatibility of electrospun polyethylene terephthalate (PET) and polyurethane (PU) scaffolds, and determined the effects on cell attachment by conditioning the fibers with adhesion proteins. Rat mesenchymal stromal cells (MSCs) were seeded on either PET or PU fiber-layered culture plates coated with laminin, collagen I. fibronectin, poly-D-lysine or gelatin. Cell density, proliferation, viability, morphology and mRNA expression were evaluated. MSC cultures on PET and PU resulted in similar cell densities and amounts of proliferating cells, with retained MSC phenotype compared to data obtained from tissue culture plate cultures. Coating the scaffolds with adhesion proteins did not increase cell density or cell proliferation. Our data suggest that both PET and PU mats, matching the dimensions of ECM fibers, are biomimetic scaffolds and, because of their high surface area-to-volume provided by the electrospinning procedure, makes them per se suitable for cell attachment and proliferation without any additional coating. (C) 2012 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalBiomaterials
Volume33
Issue number32
Pages (from-to)8094-8103
ISSN0142-9612
DOIs
Publication statusPublished - 2012
Externally publishedYes

Keywords

  • Biomaterials
  • Bioengineering
  • Ceramics and Composites
  • Mechanics of Materials
  • Biophysics
  • Biocompatible materials
  • Cell adhesion
  • Coated materials
  • Extracellular matrix
  • Fibers
  • Mesenchymal stromal cells
  • Adhesion proteins
  • Biomimetic scaffolds
  • Cell attachments
  • Cell density
  • Collagen I
  • Culture plates
  • Electrospuns
  • Extracellular matrices
  • In-vitro
  • Laminin
  • Morphology and size
  • mRNA expression
  • Native extracellular matrix
  • Poly d lysines
  • Proliferating cells
  • Three-dimensional structure
  • Tissue culture plates
  • Amino acids
  • Biocompatibility
  • Biomimetics
  • Cell proliferation
  • Coatings
  • Electrospinning
  • Flowcharting
  • Grafts
  • Morphology
  • Plates (structural components)
  • Polyethylene terephthalates
  • Proteins
  • Rats
  • Stem cells
  • Tissue
  • Tissue culture
  • Scaffolds (biology)
  • biomaterial
  • collagen type 1
  • fibronectin
  • gelatin
  • laminin
  • messenger RNA
  • poly dextro lysine
  • polyethylene terephthalate
  • polyurethan
  • tissue scaffold
  • animal cell
  • article
  • biocompatibility
  • biomimetics
  • cell adhesion
  • cell density
  • cell proliferation
  • cell structure
  • cell viability
  • controlled study
  • electrospinning
  • extracellular matrix
  • in vitro study
  • male
  • mesenchymal stroma cell
  • nonhuman
  • phenotype
  • priority journal
  • protein expression
  • rat
  • tissue culture
  • Animals
  • Cell Adhesion
  • Cell Proliferation
  • Cell Survival
  • Cells, Cultured
  • Coated Materials, Biocompatible
  • Extracellular Matrix
  • Male
  • Mesenchymal Stromal Cells
  • Polyethylene Terephthalates
  • Polyurethanes
  • Rats, Inbred Lew
  • RNA, Messenger
  • Tissue Engineering
  • Tissue Scaffolds
  • Rattus
  • ENGINEERING,
  • MATERIALS
  • MESENCHYMAL STEM-CELLS
  • EXTRACELLULAR-MATRIX
  • GROWTH-FACTORS
  • STROMAL CELLS
  • DIFFERENTIATION
  • AIRWAY
  • EXPRESSION
  • GRAFTS
  • TUMORS
  • biomimetic scaffold
  • cell attachment
  • synthetic POSS-PCU nanocomposite construct
  • Rodentia Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Rodents, Vertebrates) - Muridae [86375] rat common
  • adhesion protein
  • collagen I
  • messenger RNA mRNA expression
  • poly-D-lysine 26913-90-6
  • polyethylene terephthalate PET 25038-59-9
  • polymer 3520
  • polyurethane
  • 02506, Cytology - Animal
  • 03502, Genetics - General
  • 03506, Genetics - Animal
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10064, Biochemistry studies - Proteins, peptides and amino acids
  • 10511, Biophysics - Bioengineering
  • 32500, Tissue culture, apparatus, methods and media
  • Biochemistry and Molecular Biophysics
  • autologous stem cell
  • mesenchymal stromal cell
  • electrospinning laboratory techniques
  • tissue culture laboratory techniques, culturing techniques
  • tissue engineering laboratory techniques, culturing techniques
  • Methods and Techniques
  • Molecular Genetics

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