Venous responses during exercise in rainbow trout, Oncorhynchus mykiss : [alpha]-adrenergic control and the antihypotensive function of the renin-angiotensin system

E. Sandblom, M. Axelsson, David McKenzie

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Abstract

The role of the [alpha]-adrenergic system in the control of cardiac preload (central venous blood pressure; Pven) and venous capacitance during exercise was investigated in rainbow trout (Oncorhynchus mykiss). In addition, the antihypotensive effect of the renin-angiotesin system (RAS) was investigated during exercise after [alpha]-adrenoceptor blockade. Fish were subjected to a 20-min exercise challenge at 0.66 body lengths s-1 (BL s-1) while Pven, dorsal aortic blood pressure (Pda) and relative cardiac output (Q) was recorded continuously. Heart rate (fH), cardiac stroke volume (SV) and total systemic resistance (Rsys) were derived from these variables. The mean circulatory filling pressure (MCFP) was measured at rest and at the end of the exercise challenge, to investigate potential exercise-mediated changes in venous capacitance. The protocol was repeated after [alpha]-adrenoceptor blockade with prazosin (1[no-break space]mg kg-1 Mb) and again after additional blockade of angiotensin converting enzyme (ACE) with enalapril (1[no-break space]mg kg-1 Mb). In untreated fish, exercise was associated with a rapid (within approx. 1-2[no-break space]min) and sustained increase in Q and Pven associated with a significant increase in MCFP (0.17 +/- 0.02[no-break space]kPa at rest to 0.27 +/- 0.02[no-break space]kPa at the end of exercise). Prazosin treatment did not block the exercise-mediated increase in MCFP (0.25 +/- 0.04[no-break space]kPa to 0.33 +/- 0.04[no-break space]kPa at the end of exercise), but delayed the other cardiovascular responses to swimming such that Q and Pven did not increase significantly until around 10-13[no-break space]min of exercise, suggesting that an endogenous humoral control mechanism had been activated. Subsequent enalapril treatment revealed that these delayed responses were in fact due to activation of the RAS, because resting Pda and Rsys were decreased further and essentially all cardiovascular changes during exercise were abolished. This study shows that the [alpha]-adrenergic system normally plays an important role in the control of venous function during exercise in rainbow trout. It is also the first study to suggest that the RAS may be an important modulator of venous pressure and capacitance in fish.
Original languageEnglish
JournalComparative Biochemistry and Physiology A-Molecular & Integrative Physiology
Volume144
Issue number4
Pages (from-to)401-409
ISSN1095-6433
Publication statusPublished - 2006

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