Validation of real time PCR assays for use in routine diagnostics of pig diarrhoea

Marie Ståhl; Charlotte Kristiane Hjulsager; Solvej Østergaard Breum; Lars Erik Larsen; Sven Erik Lind Jorsal; Øystein Angen

Validation of real time PCR assays for use in routine diagnostics of pig diarrhoea

Marie Ståhl, Charlotte Kristiane Hjulsager, Solvej Østergaard Breum, Lars Erik Larsen, Sven Erik Lind Jorsal, Øystein Angen

Research output: Contribution to conference › Poster › Research › peer-review

Abstract

At the National Veterinary Institute in Denmark we want to optimize routine diagnostic analyses by screening samples simultaneously for several agents by real time PCR. Here we present the validation of real time PCR assays for E. coli F4. E coli F18 and Lawsonia intracellularis2 in pig feces. The validation is based on feces samples spiked with a serial dilution of the respective bacteria for determination of PCR efficiencies, dynamic ranges and detection limits. In addition, the effect of the PCR assays of different concentrations of feces and pig to pig variation have been evaluated.

Original language	English
Publication date	2008
Publication status	Published - 2008
Event	Advances in qPCR - Stockholm, Sweden Duration: 17 Sep 2008 → 18 Sep 2008

Conference

Conference	Advances in qPCR
Country/Territory	Sweden
City	Stockholm
Period	17/09/2008 → 18/09/2008

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title = "Validation of real time PCR assays for use in routine diagnostics of pig diarrhoea",

abstract = "At the National Veterinary Institute in Denmark we want to optimize routine diagnostic analyses by screening samples simultaneously for several agents by real time PCR. Here we present the validation of real time PCR assays for E. coli F4. E coli F18 and Lawsonia intracellularis2 in pig feces. The validation is based on feces samples spiked with a serial dilution of the respective bacteria for determination of PCR efficiencies, dynamic ranges and detection limits. In addition, the effect of the PCR assays of different concentrations of feces and pig to pig variation have been evaluated.",

author = "Marie St{\aa}hl and Hjulsager, {Charlotte Kristiane} and Breum, {Solvej {\O}stergaard} and Larsen, {Lars Erik} and Jorsal, {Sven Erik Lind} and {\O}ystein Angen",

year = "2008",

language = "English",

note = "Advances in qPCR ; Conference date: 17-09-2008 Through 18-09-2008",

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Validation of real time PCR assays for use in routine diagnostics of pig diarrhoea. / Ståhl, Marie; Hjulsager, Charlotte Kristiane; Breum, Solvej Østergaard; Larsen, Lars Erik; Jorsal, Sven Erik Lind; Angen, Øystein.

2008. Poster session presented at Advances in qPCR, Stockholm, Sweden.

Research output: Contribution to conference › Poster › Research › peer-review

TY - CONF

T1 - Validation of real time PCR assays for use in routine diagnostics of pig diarrhoea

AU - Ståhl, Marie

AU - Hjulsager, Charlotte Kristiane

AU - Breum, Solvej Østergaard

AU - Larsen, Lars Erik

AU - Jorsal, Sven Erik Lind

AU - Angen, Øystein

PY - 2008

Y1 - 2008

N2 - At the National Veterinary Institute in Denmark we want to optimize routine diagnostic analyses by screening samples simultaneously for several agents by real time PCR. Here we present the validation of real time PCR assays for E. coli F4. E coli F18 and Lawsonia intracellularis2 in pig feces. The validation is based on feces samples spiked with a serial dilution of the respective bacteria for determination of PCR efficiencies, dynamic ranges and detection limits. In addition, the effect of the PCR assays of different concentrations of feces and pig to pig variation have been evaluated.

AB - At the National Veterinary Institute in Denmark we want to optimize routine diagnostic analyses by screening samples simultaneously for several agents by real time PCR. Here we present the validation of real time PCR assays for E. coli F4. E coli F18 and Lawsonia intracellularis2 in pig feces. The validation is based on feces samples spiked with a serial dilution of the respective bacteria for determination of PCR efficiencies, dynamic ranges and detection limits. In addition, the effect of the PCR assays of different concentrations of feces and pig to pig variation have been evaluated.

M3 - Poster

T2 - Advances in qPCR

Y2 - 17 September 2008 through 18 September 2008

ER -

Validation of real time PCR assays for use in routine diagnostics of pig diarrhoea

Abstract

Conference

UN SDGs

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