Validation of real time PCR assays for use in routine diagnostics of pig diarrhoea

Marie Ståhl, Charlotte Kristiane Hjulsager, Solvej Østergaard Breum, Lars Erik Larsen, Sven Erik Lind Jorsal, Øystein Angen

    Research output: Contribution to conferencePosterResearchpeer-review

    Abstract

    At the National Veterinary Institute in Denmark we want to optimize routine diagnostic analyses by screening samples simultaneously for several agents by real time PCR. Here we present the validation of real time PCR assays for E. coli F4. E coli F18 and Lawsonia intracellularis2 in pig feces. The validation is based on feces samples spiked with a serial dilution of the respective bacteria for determination of PCR efficiencies, dynamic ranges and detection limits. In addition, the effect of the PCR assays of different concentrations of feces and pig to pig variation have been evaluated.
    Original languageEnglish
    Publication date2008
    Publication statusPublished - 2008
    EventAdvances in qPCR - Stockholm, Sweden
    Duration: 17 Sep 200818 Sep 2008

    Conference

    ConferenceAdvances in qPCR
    CountrySweden
    CityStockholm
    Period17/09/200818/09/2008

    Cite this

    Ståhl, M., Hjulsager, C. K., Breum, S. Ø., Larsen, L. E., Jorsal, S. E. L., & Angen, Ø. (2008). Validation of real time PCR assays for use in routine diagnostics of pig diarrhoea. Poster session presented at Advances in qPCR, Stockholm, Sweden.