At the National Veterinary Institute in Denmark we want to optimize routine diagnostic analyses by screening samples simultaneously for several agents by real time PCR. Here we present the validation of real time PCR assays for E. coli F4. E coli F18 and Lawsonia intracellularis2 in pig feces. The validation is based on feces samples spiked with a serial dilution of the respective bacteria for determination of PCR efficiencies, dynamic ranges and detection limits. In addition, the effect of the PCR assays of different concentrations of feces and pig to pig variation have been evaluated.
|Publication status||Published - 2008|
|Event||Advances in qPCR - Stockholm, Sweden|
Duration: 17 Sep 2008 → 18 Sep 2008
|Conference||Advances in qPCR|
|Period||17/09/2008 → 18/09/2008|
Ståhl, M., Hjulsager, C. K., Breum, S. Ø., Larsen, L. E., Jorsal, S. E. L., & Angen, Ø. (2008). Validation of real time PCR assays for use in routine diagnostics of pig diarrhoea. Poster session presented at Advances in qPCR, Stockholm, Sweden.