Validation of QuEChERS for screening of 4 marker polycyclic aromatic hydrocarbons in fish and malt

Lene Duedahl-Olesen*, N. M. Iversen, Catharina Kelmo, Lisbeth Krüger Jensen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Application of QuEChERS as a new quick and easy method for screening of polycyclic aromatic hydrocarbons (PAHs) was developed, validated and used for quantification (GC-QTOF-MS) of 4 PAHs, namely benz[a]anthracene, chrysene, benzo[b]fluoranthene and benzo[a]pyrene. A clean-up step using Zirconium based dispersive SPE was applied. Validation based on spiked samples (0.5, 1 and 4 μg/kg) revealed a limit of quantification (LOQ) for the 4 PAH compounds in the range of 0.14–0.24 μg/kg for fish and 0.12–0.24 μg/kg for raw barley. Overall recoveries ranged from 88 to 117% with repeatability and in-house reproducibility from 2.6 to 16%. The method was applied to 9 malt samples which were found to contain the sum of 4 PAH from <0.36 μg/kg for raw barley to 26 μg/kg for peat smoked barley. For 5 smoked fish samples the sum of PAH4 ranged from <0.34 μg/kg for cold smoked salmon to 2.2 μg/kg for hot smoked mackerel. In general it was found that the method could be used as a fast screening for evaluation of smoked fish according to EU Commission maximum concentrations of benzo[a]pyrene (2.0 μg/kg) and the sum of PAH4 (10 μg/kg).

Original languageEnglish
Article number106434
JournalFood Control
Volume108
Number of pages7
ISSN0956-7135
DOIs
Publication statusPublished - 2020

Keywords

  • benzo[a]pyrene
  • Fish
  • GC-Q-TOF-MS
  • Malt
  • PAH4
  • QuEChERS

Cite this

@article{402f9351c1934d3daeae19333fdd454e,
title = "Validation of QuEChERS for screening of 4 marker polycyclic aromatic hydrocarbons in fish and malt",
abstract = "Application of QuEChERS as a new quick and easy method for screening of polycyclic aromatic hydrocarbons (PAHs) was developed, validated and used for quantification (GC-QTOF-MS) of 4 PAHs, namely benz[a]anthracene, chrysene, benzo[b]fluoranthene and benzo[a]pyrene. A clean-up step using Zirconium based dispersive SPE was applied. Validation based on spiked samples (0.5, 1 and 4 μg/kg) revealed a limit of quantification (LOQ) for the 4 PAH compounds in the range of 0.14–0.24 μg/kg for fish and 0.12–0.24 μg/kg for raw barley. Overall recoveries ranged from 88 to 117{\%} with repeatability and in-house reproducibility from 2.6 to 16{\%}. The method was applied to 9 malt samples which were found to contain the sum of 4 PAH from <0.36 μg/kg for raw barley to 26 μg/kg for peat smoked barley. For 5 smoked fish samples the sum of PAH4 ranged from <0.34 μg/kg for cold smoked salmon to 2.2 μg/kg for hot smoked mackerel. In general it was found that the method could be used as a fast screening for evaluation of smoked fish according to EU Commission maximum concentrations of benzo[a]pyrene (2.0 μg/kg) and the sum of PAH4 (10 μg/kg).",
keywords = "benzo[a]pyrene, Fish, GC-Q-TOF-MS, Malt, PAH4, QuEChERS",
author = "Lene Duedahl-Olesen and Iversen, {N. M.} and Catharina Kelmo and {Kr{\"u}ger Jensen}, Lisbeth",
year = "2020",
doi = "10.1016/j.foodcont.2018.12.010",
language = "English",
volume = "108",
journal = "Food Control",
issn = "0956-7135",
publisher = "Pergamon Press",

}

Validation of QuEChERS for screening of 4 marker polycyclic aromatic hydrocarbons in fish and malt. / Duedahl-Olesen, Lene; Iversen, N. M.; Kelmo, Catharina; Krüger Jensen, Lisbeth.

In: Food Control, Vol. 108, 106434, 2020.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Validation of QuEChERS for screening of 4 marker polycyclic aromatic hydrocarbons in fish and malt

AU - Duedahl-Olesen, Lene

AU - Iversen, N. M.

AU - Kelmo, Catharina

AU - Krüger Jensen, Lisbeth

PY - 2020

Y1 - 2020

N2 - Application of QuEChERS as a new quick and easy method for screening of polycyclic aromatic hydrocarbons (PAHs) was developed, validated and used for quantification (GC-QTOF-MS) of 4 PAHs, namely benz[a]anthracene, chrysene, benzo[b]fluoranthene and benzo[a]pyrene. A clean-up step using Zirconium based dispersive SPE was applied. Validation based on spiked samples (0.5, 1 and 4 μg/kg) revealed a limit of quantification (LOQ) for the 4 PAH compounds in the range of 0.14–0.24 μg/kg for fish and 0.12–0.24 μg/kg for raw barley. Overall recoveries ranged from 88 to 117% with repeatability and in-house reproducibility from 2.6 to 16%. The method was applied to 9 malt samples which were found to contain the sum of 4 PAH from <0.36 μg/kg for raw barley to 26 μg/kg for peat smoked barley. For 5 smoked fish samples the sum of PAH4 ranged from <0.34 μg/kg for cold smoked salmon to 2.2 μg/kg for hot smoked mackerel. In general it was found that the method could be used as a fast screening for evaluation of smoked fish according to EU Commission maximum concentrations of benzo[a]pyrene (2.0 μg/kg) and the sum of PAH4 (10 μg/kg).

AB - Application of QuEChERS as a new quick and easy method for screening of polycyclic aromatic hydrocarbons (PAHs) was developed, validated and used for quantification (GC-QTOF-MS) of 4 PAHs, namely benz[a]anthracene, chrysene, benzo[b]fluoranthene and benzo[a]pyrene. A clean-up step using Zirconium based dispersive SPE was applied. Validation based on spiked samples (0.5, 1 and 4 μg/kg) revealed a limit of quantification (LOQ) for the 4 PAH compounds in the range of 0.14–0.24 μg/kg for fish and 0.12–0.24 μg/kg for raw barley. Overall recoveries ranged from 88 to 117% with repeatability and in-house reproducibility from 2.6 to 16%. The method was applied to 9 malt samples which were found to contain the sum of 4 PAH from <0.36 μg/kg for raw barley to 26 μg/kg for peat smoked barley. For 5 smoked fish samples the sum of PAH4 ranged from <0.34 μg/kg for cold smoked salmon to 2.2 μg/kg for hot smoked mackerel. In general it was found that the method could be used as a fast screening for evaluation of smoked fish according to EU Commission maximum concentrations of benzo[a]pyrene (2.0 μg/kg) and the sum of PAH4 (10 μg/kg).

KW - benzo[a]pyrene

KW - Fish

KW - GC-Q-TOF-MS

KW - Malt

KW - PAH4

KW - QuEChERS

U2 - 10.1016/j.foodcont.2018.12.010

DO - 10.1016/j.foodcont.2018.12.010

M3 - Journal article

AN - SCOPUS:85072217660

VL - 108

JO - Food Control

JF - Food Control

SN - 0956-7135

M1 - 106434

ER -