Validation of in vitro probabilistic tractography

Tim B. Dyrby, L.V. Sogaard, G.J. Parker, D.C. Alexander, N.M. Lind, W.F.C. Baare, A. Hay-Schmidt, N. Eriksen, B. Pakkenberg, O.B. Paulson, J. Jelsing

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Diffusion weighted imaging (DWI) and tractography allow the non-invasive study of anatomical brain connectivity. However, a gold standard for validating tractography of complex connections is lacking. Using the porcine brain as a highly gyrated brain model, we quantitatively and qualitatively assessed the anatomical validity and reproducibility of in vitro multi-fiber probabilistic tractography against two invasive tracers: the histochemically detectable biotinylated dextran amine and manganese enhanced magnetic resonance imaging. Post mortern DWI was used to ensure that most of the sources known to degrade the anatomical accuracy of in vivo DWI did not influence the tracking results. We demonstrate that probabilistic tractography reliably detected specific pathways. Moreover, the applied model allowed identification of the limitations that are likely to appear in many of the current tractography methods. Nevertheless, we conclude that DWI tractography can be a precise tool in studying anatomical brain connectivity.
    Original languageEnglish
    JournalNeuroImage
    Volume37
    Issue number4
    Pages (from-to)1267-1277
    ISSN1053-8119
    DOIs
    Publication statusPublished - 2007

    Keywords

    • neuron tracing
    • post mortem
    • diffusion weighted imaging
    • manganese
    • brain
    • multi-fiber
    • Gottingen minipig

    Fingerprint Dive into the research topics of 'Validation of in vitro probabilistic tractography'. Together they form a unique fingerprint.

    Cite this