Validation of a PCR-based method for detection of food-borne thermotolerant Campylobacters in a multicenter collaborative trial

Mathilde Hartmann Josefsen, N. Cook, M. D'Agostino, F. Hansen, M. Wagner, K. Demnerova, A.E. Heuvelink, P.T. Tassios, H. Lindmark, V. Kmet, M. Barbanera, P. Fach, S. Loncarevic, Jeffrey Hoorfar

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A PCR-based method for rapid detection of food-borne thermotolerant campylobacters was evaluated through a collaborative trial with 12 laboratories testing spiked carcass rinse samples. The method showed an interlaboratory diagnostic sensitivity of 96.7% and a diagnostic specificity of 100% for chicken samples, while these values were 94.2 and 83.3%, respectively, for pig samples.
Original languageEnglish
JournalApplied and Environmental Microbiology
Volume70
Issue number7
Pages (from-to)4379-4383
ISSN0099-2240
DOIs
Publication statusPublished - 2004

Cite this

Josefsen, Mathilde Hartmann ; Cook, N. ; D'Agostino, M. ; Hansen, F. ; Wagner, M. ; Demnerova, K. ; Heuvelink, A.E. ; Tassios, P.T. ; Lindmark, H. ; Kmet, V. ; Barbanera, M. ; Fach, P. ; Loncarevic, S. ; Hoorfar, Jeffrey. / Validation of a PCR-based method for detection of food-borne thermotolerant Campylobacters in a multicenter collaborative trial. In: Applied and Environmental Microbiology. 2004 ; Vol. 70, No. 7. pp. 4379-4383.
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title = "Validation of a PCR-based method for detection of food-borne thermotolerant Campylobacters in a multicenter collaborative trial",
abstract = "A PCR-based method for rapid detection of food-borne thermotolerant campylobacters was evaluated through a collaborative trial with 12 laboratories testing spiked carcass rinse samples. The method showed an interlaboratory diagnostic sensitivity of 96.7{\%} and a diagnostic specificity of 100{\%} for chicken samples, while these values were 94.2 and 83.3{\%}, respectively, for pig samples.",
author = "Josefsen, {Mathilde Hartmann} and N. Cook and M. D'Agostino and F. Hansen and M. Wagner and K. Demnerova and A.E. Heuvelink and P.T. Tassios and H. Lindmark and V. Kmet and M. Barbanera and P. Fach and S. Loncarevic and Jeffrey Hoorfar",
year = "2004",
doi = "10.1128/AEM.70.7.4379-4383.2004",
language = "English",
volume = "70",
pages = "4379--4383",
journal = "Applied and Environmental Microbiology",
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Josefsen, MH, Cook, N, D'Agostino, M, Hansen, F, Wagner, M, Demnerova, K, Heuvelink, AE, Tassios, PT, Lindmark, H, Kmet, V, Barbanera, M, Fach, P, Loncarevic, S & Hoorfar, J 2004, 'Validation of a PCR-based method for detection of food-borne thermotolerant Campylobacters in a multicenter collaborative trial', Applied and Environmental Microbiology, vol. 70, no. 7, pp. 4379-4383. https://doi.org/10.1128/AEM.70.7.4379-4383.2004

Validation of a PCR-based method for detection of food-borne thermotolerant Campylobacters in a multicenter collaborative trial. / Josefsen, Mathilde Hartmann; Cook, N.; D'Agostino, M.; Hansen, F.; Wagner, M.; Demnerova, K.; Heuvelink, A.E.; Tassios, P.T.; Lindmark, H.; Kmet, V.; Barbanera, M.; Fach, P.; Loncarevic, S.; Hoorfar, Jeffrey.

In: Applied and Environmental Microbiology, Vol. 70, No. 7, 2004, p. 4379-4383.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Validation of a PCR-based method for detection of food-borne thermotolerant Campylobacters in a multicenter collaborative trial

AU - Josefsen, Mathilde Hartmann

AU - Cook, N.

AU - D'Agostino, M.

AU - Hansen, F.

AU - Wagner, M.

AU - Demnerova, K.

AU - Heuvelink, A.E.

AU - Tassios, P.T.

AU - Lindmark, H.

AU - Kmet, V.

AU - Barbanera, M.

AU - Fach, P.

AU - Loncarevic, S.

AU - Hoorfar, Jeffrey

PY - 2004

Y1 - 2004

N2 - A PCR-based method for rapid detection of food-borne thermotolerant campylobacters was evaluated through a collaborative trial with 12 laboratories testing spiked carcass rinse samples. The method showed an interlaboratory diagnostic sensitivity of 96.7% and a diagnostic specificity of 100% for chicken samples, while these values were 94.2 and 83.3%, respectively, for pig samples.

AB - A PCR-based method for rapid detection of food-borne thermotolerant campylobacters was evaluated through a collaborative trial with 12 laboratories testing spiked carcass rinse samples. The method showed an interlaboratory diagnostic sensitivity of 96.7% and a diagnostic specificity of 100% for chicken samples, while these values were 94.2 and 83.3%, respectively, for pig samples.

U2 - 10.1128/AEM.70.7.4379-4383.2004

DO - 10.1128/AEM.70.7.4379-4383.2004

M3 - Journal article

VL - 70

SP - 4379

EP - 4383

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 7

ER -