Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)

S M Bergmann; Q Wang; W Zeng; Y Li; Y Wang; M Matras; M. Reichert; D. Fichtner; M. Lenk; T. Morin; Niels Jørgen Olesen; Helle Frank Skall; P.-Y. Lee; S. Zheng; S. Monaghan; S. Reiche; W. Fuchs; M. Kotler; K. Way; G. Bräuer; K. Böttcher; A. Kappe; J. Kielpinska

doi:10.1111/jfd.12621

Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)

S M Bergmann, Q Wang, W Zeng, Y Li, Y Wang, M Matras, M. Reichert, D. Fichtner, M. Lenk, T. Morin, Niels Jørgen Olesen, Helle Frank Skall, P.-Y. Lee, S. Zheng, S. Monaghan, S. Reiche, W. Fuchs, M. Kotler, K. Way, G. BräuerK. Böttcher, A. Kappe, J. Kielpinska

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.

Original language	English
Journal	Journal of Fish Diseases
Volume	40
Issue number	11
Pages (from-to)	1511-1527
ISSN	0140-7775
DOIs	https://doi.org/10.1111/jfd.12621
Publication status	Published - 2017

Keywords

Carp and koi sera
Enzyme-linked immunosorbent assay
Koi herpesvirus
Koi herpesvirus antibodies

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10.1111/jfd.12621

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Bergmann, S. M., Wang, Q., Zeng, W., Li, Y., Wang, Y., Matras, M., Reichert, M., Fichtner, D., Lenk, M., Morin, T., Olesen, N. J., Skall, H. F., Lee, P-Y., Zheng, S., Monaghan, S., Reiche, S., Fuchs, W., Kotler, M., Way, K., ... Kielpinska, J. (2017). Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA). Journal of Fish Diseases, 40(11), 1511-1527. https://doi.org/10.1111/jfd.12621

@article{7545439919e647138e5e3eba6ab0d442,

title = "Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)",

abstract = "Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.",

keywords = "Carp and koi sera, Enzyme-linked immunosorbent assay, Koi herpesvirus, Koi herpesvirus antibodies",

author = "Bergmann, {S M} and Q Wang and W Zeng and Y Li and Y Wang and M Matras and M. Reichert and D. Fichtner and M. Lenk and T. Morin and Olesen, {Niels J{\o}rgen} and Skall, {Helle Frank} and P.-Y. Lee and S. Zheng and S. Monaghan and S. Reiche and W. Fuchs and M. Kotler and K. Way and G. Br{\"a}uer and K. B{\"o}ttcher and A. Kappe and J. Kielpinska",

year = "2017",

doi = "10.1111/jfd.12621",

language = "English",

volume = "40",

pages = "1511--1527",

journal = "Journal of Fish Diseases",

issn = "0140-7775",

publisher = "Wiley-Blackwell",

number = "11",

}

Bergmann, SM, Wang, Q, Zeng, W, Li, Y, Wang, Y, Matras, M, Reichert, M, Fichtner, D, Lenk, M, Morin, T, Olesen, NJ, Skall, HF, Lee, P-Y, Zheng, S, Monaghan, S, Reiche, S, Fuchs, W, Kotler, M, Way, K, Bräuer, G, Böttcher, K, Kappe, A & Kielpinska, J 2017, 'Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)', Journal of Fish Diseases, vol. 40, no. 11, pp. 1511-1527. https://doi.org/10.1111/jfd.12621

TY - JOUR

T1 - Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)

AU - Bergmann, S M

AU - Wang, Q

AU - Zeng, W

AU - Li, Y

AU - Wang, Y

AU - Matras, M

AU - Reichert, M.

AU - Fichtner, D.

AU - Lenk, M.

AU - Morin, T.

AU - Olesen, Niels Jørgen

AU - Skall, Helle Frank

AU - Lee, P.-Y.

AU - Zheng, S.

AU - Monaghan, S.

AU - Reiche, S.

AU - Fuchs, W.

AU - Kotler, M.

AU - Way, K.

AU - Bräuer, G.

AU - Böttcher, K.

AU - Kappe, A.

AU - Kielpinska, J.

PY - 2017

Y1 - 2017

N2 - Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.

AB - Koi herpesvirus (KHV) causes KHV disease (KHVD). The virus is highly contagious in carp or koi and can induce a high mortality. Latency and, in some cases, a lack of signs presents a challenge for virus detection. Appropriate immunological detection methods for anti-KHV antibodies have not yet been fully validated for KHV. Therefore, it was developed and validated an enzyme-linked immunosorbent assay (ELISA) to detect KHV antibodies. The assay was optimized with respect to plates, buffers, antigens and assay conditions. It demonstrated high diagnostic and analytical sensitivity and specificity and was particularly useful at the pond or farm levels. Considering the scale of the carp and koi industry worldwide, this assay represents an important practical tool for the indirect detection of KHV, also in the absence of clinical signs.

KW - Carp and koi sera

KW - Enzyme-linked immunosorbent assay

KW - Koi herpesvirus

KW - Koi herpesvirus antibodies

U2 - 10.1111/jfd.12621

DO - 10.1111/jfd.12621

M3 - Journal article

C2 - 28470973

SN - 0140-7775

VL - 40

SP - 1511

EP - 1527

JO - Journal of Fish Diseases

JF - Journal of Fish Diseases

IS - 11

ER -

Validation of a KHV antibody enzyme-linked immunosorbent assay (ELISA)

Abstract

Keywords

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