Validation of a Diagnostic PCR Method for Routine Analysis of Salmonella spp. in Animal Feed Samples

Charlotta Löfström, Charlotta Engdahl Axelsson, Peter Radstrom

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

As a part of a validation study, a comparative study of a PCR method and the standard culture-based method NMKL-71, for detection of Salmonella, was performed according to the validation protocol from the Nordic validation organ for validation of alternative microbiological methods (NordVal) on 250 artificially or naturally contaminated animal feed samples. The PCR method is based on culture enrichment in buffered peptone water followed by PCR using the DNA polymerase Tth and an internal amplification control. No significant difference was found between the two methods. The relative accuracy, relative sensitivity and relative specificity were found to be 96.0, 97.3, and 98.8%, respectively. PCR inhibition was observed for rape seed samples. For the acidified feed samples, more Salmonella-positive samples were found with the PCR method compared to the NMKL method. This study focuses on the growing demand for validated diagnostic PCR methods for routine analysis of animal feed and food samples to assure safety in the food production chain.
Original languageEnglish
JournalFood Analytical Methods
Volume1
Issue number1
Pages (from-to)23-27
Number of pages5
ISSN1936-9751
DOIs
Publication statusPublished - 2008
Externally publishedYes

Keywords

  • Animal Feed
  • NordVal
  • Salmonella
  • Validation
  • PCR
  • Polymerase Chain Reaction
  • FOOD
  • REAL-TIME PCR
  • POLYMERASE-CHAIN-REACTION
  • AMPLIFICATION
  • ENRICHMENT
  • SYSTEM
  • ASSAY
  • CELL CULTURE
  • CULTURE
  • FEEDS
  • FOOD SAFETY
  • GENETIC TECHNIQUES
  • SALMONELLA
  • Hygiene and toxicology
  • Microbiological aspects

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