Vaccination of mice with plasmids expressing processed capsid protein of foot-and-mouth disease virus - Importance of dominant and subdominant epitopes for antigenicity and protection

Tine Frimann, Annette Malene Barfoed, Bent Aasted, Søren Kamstrup

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The capsid of foot-and-mouth disease virus (FMDV) displays several independent B cell epitopes, which stimulate the production of neutralising antibodies. Some of these epitopes are highly variable between virus strains, but dominate the immune response. The site A on VP1 is the most prominent example of a dominant and variable site. This variability is a problem when designing vaccines against this disease, because it necessitates a close match between vaccine strain and virus in an outbreak. We have introduced a series of mutations into viral capsid proteins with the aim of selectively silencing two dominant and highly variable epitopes and thereby divert immune responses toward less dominant but more conserved, protective epitopes. When mice were immunized with modified antigens, the resulting immune responses showed a higher degree of cross-reactivity towards heterologous virus as compared to mice vaccinated with wild type epitopes. Most of the modifications did not adversely affect the ability of the plasmids to induce complete protection of mice against homologous challenge.
    Original languageEnglish
    JournalVaccine
    Volume25
    Issue number33
    Pages (from-to)6191-6200
    ISSN0264-410X
    DOIs
    Publication statusPublished - 2007

    Keywords

    • protection
    • cross-reactivity
    • B-cell epitopes
    • FMDV
    • foot-and-mouth disease

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