UV-guided isolation of fungal metabolites by HSCCC

P.W. Dalsgaard, K.F. Nielsen, Thomas Ostenfeld Larsen

    Research output: Contribution to journalJournal articleResearchpeer-review


    Analytical standardised reversed phase liquid chromatography (RPLC) data can be helpful in finding a suitable solvent combination for isolation of fungal metabolites by high-speed counter current chromatography. Analysis of the distribution coefficient (K-D) of fungal metabolites in a series of biphasic systems consisting of n-heptane-ethyl acetate (EtOAc)-methanol (MeOH)-water has been used to illustrate the relationship between the RPLC retention indexes and K-D's. The relationship can be used to select a suitable solvent combination for HSCCC, as demonstrated by the isolation of two new cyclic peptides from a crude fungal extract.
    Original languageEnglish
    JournalJournal of Liquid Chromatography & Related Technologies
    Issue number12-13
    Pages (from-to)2029-2039
    Publication statusPublished - 2005


    • alkylphenones
    • LC-MS
    • Penicillium rivulum
    • dereplication
    • Margraff system
    • CCC
    • filamentous fungi
    • LECA
    • psychrophilin

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