Abstract
Analytical standardised reversed phase liquid chromatography (RPLC) data can be helpful in finding a suitable solvent combination for isolation of fungal metabolites by high-speed counter current chromatography. Analysis of the distribution coefficient (K-D) of fungal metabolites in a series of biphasic systems consisting of n-heptane-ethyl acetate (EtOAc)-methanol (MeOH)-water has been used to illustrate the relationship between the RPLC retention indexes and K-D's. The relationship can be used to select a suitable solvent combination for HSCCC, as demonstrated by the isolation of two new cyclic peptides from a crude fungal extract.
| Original language | English |
|---|---|
| Journal | Journal of Liquid Chromatography & Related Technologies |
| Volume | 28 |
| Issue number | 12-13 |
| Pages (from-to) | 2029-2039 |
| ISSN | 1082-6076 |
| Publication status | Published - 2005 |
Keywords
- alkylphenones
- LC-MS
- Penicillium rivulum
- dereplication
- Margraff system
- CCC
- filamentous fungi
- LECA
- psychrophilin