Using Impedance Flow Cytometry for Rapid Viability Classification of Heat-Treated Bacteria

Christian Vinther Bertelsen*, Gustav Erik Skands, Marcos González Díaz, Maria Dimaki, Winnie Edith Svendsen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

In the future, rapid electrical characterization of cells with impedance flow cytometry promises to be a fast and accurate method for the evaluation of cell properties. In this paper, we investigate how the conductivity of the suspending medium along with the heat exposure time affects the viability classification of heat-treated E. coli. Using a theoretical model, we show that perforation of the bacteria membrane during heat exposure changes the impedance of the bacterial cell from effectively less conducting than the suspension medium to effectively more conducting. Consequently, this results in a shift in the differential argument of the complex electrical current that can be measured with impedance flow cytometry. We observe this shift experimentally through measurements on E. coli samples with varying medium conductivity and heat exposure times. We show that increased exposure time and lower medium conductivity results in improved classification between untreated and heat-treated bacteria. The best classification was achieved with a medium conductivity of 0.045 S/m after 30 min of heat exposure.
Original languageEnglish
JournalACS Omega
Volume8
Issue number8
Pages (from-to)7714-7721
Number of pages8
DOIs
Publication statusPublished - 2023

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