Use of self‐assembled monolayers for the sequential and independent immobilisation of enzymes

Edmond Magner*, Alessandro Serleti, Xinxin Xiao, Kim Shortall

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

The precise spatial control of the immobilisation of enzymes is necessary for the sequential localization of an enzymatic cascade. In this contribution, the sequential immobilisation of alcohol dehydrogenase (ADH), formaldehyde dehydrogenase (FLDH) and formate dehydrogenase (FoDH) on self-assembled monolayer modified electrodes has been demonstrated. A range of thiols were screened. Optimal enzymatic activity was obtained with thiols bearing -OH, a positively charged heterocyclic aromatic ring and -COOH for the immobilisation of ADH, FLDH and FoDH, respectively. The application of a potential pulse sequence was utilised to separately modify individual electrodes with the appropriate thiol and enzyme to deliver a three enzyme system with catalytically active enzyme. The immobilised enzymes displayed a range of stability (40-70%) on storage for a period of two weeks.
Original languageEnglish
JournalChemElectroChem
ISSN2196-0216
DOIs
Publication statusAccepted/In press - 2021

Keywords

  • Self-assembled monolayers
  • Enzyme cascade
  • Gold electrode
  • Dehydrogenase
  • Sequential immobilisation

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