Use of prokaryotic transcriptional activators as metabolite biosensors in eukaryotic cells

Michael Krogh Jensen (Inventor), Jay Keasling (Inventor), Mette Louise Skjødt (Inventor), Tim Snoek (Inventor)

Research output: Patent

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Abstract

The present invention relates to the use of transcriptional activators from prokaryotic organisms for use in eukaryotic cells, such as yeast as sensors of intracellular and extracellular accumulation of a ligand or metabolite specifically activating this transcriptional activator in a eukaryot, such as yeast cell, such as a cell engineered to produce this ligand. The transcriptional activator controls a promoter upstream of one or more gene, which may include e.g. a reporter gene that may be a fluorescence marker, such as luciferase, green fluorescent protein or a gnee encoding antibiotic resistance.
Original languageEnglish
IPCC12N15/67; C12N15/81
Patent numberWO2018007526
Filing date11/01/2018
CountryInternational Bureau of the World Intellectual Property Organization (WIPO)
Priority date06/07/2016
Priority numberEP20160178220
Publication statusPublished - 2018

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