Abstract
The present invention relates to the use of transcriptional activators from prokaryotic organisms for use in eukaryotic cells, such as yeast as sensors of intracellular and extracellular accumulation of a ligand or metabolite specifically activating this transcriptional activator in a eukaryot, such as yeast cell, such as a cell engineered to produce this ligand. The transcriptional activator controls a promoter upstream of one or more gene, which may include e.g. a reporter gene that may be a fluorescence marker, such as luciferase, green fluorescent protein or a gnee encoding antibiotic resistance.
| Original language | English |
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| IPC | C12N15/67; C12N15/81 |
| Patent number | WO2018007526 |
| Filing date | 11/01/2018 |
| Country/Territory | International Bureau of the World Intellectual Property Organization (WIPO) |
| Priority date | 06/07/2016 |
| Priority number | EP20160178220 |
| Publication status | Published - 2018 |