Use of laminar flow patterning for miniaturised biochemical assays

Birgitte Regenberg, Ulrich Krühne, M. Beyer, L.H. Pedersen, Marcos Simon Soria, Owen R. T. Thomas, Jens Nielsen, T. Ahl

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Laminar flow in microfluidic chambers was used to construct low (one dimensional) density arrays suitable for miniaturized biochemical assays. By varying the ratio of flows of two guiding streams flanking a sample stream, precise focusing and positioning of the latter was achieved, and reactive species carried in the sample stream were deposited on functionalized chip surfaces as discrete 50 mm wide lanes. Using different model systems we have confirmed the method's suitability for qualitative screening and quantification tasks in receptor-ligand assays, recording biotin-streptavidin interactions, DNA-hybridization and DNA-triplex formation. The system is simple, fast, reproducible, flexible, and has small sample requirements.
Original languageEnglish
JournalLab on a Chip
Volume4
Pages (from-to)654-657
ISSN1473-0197
DOIs
Publication statusPublished - 2004

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