Use of high-gradient magnetic fishing for reducing proteolysis during fermentation.

Trine Lütken Maury, Kim Ekelund Ottow, Jesper Brask, John Villadsen, Timothy John Hobley

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Proteolysis during fermentation may have a severe impact on the yield and quality of a secreted product. In the current study, we demonstrate the use of high-gradient magnetic fishing (HGMF) as an efficient alternative to the more conventional methods of preventing proteolytic degradation. Bacitracin-linked magnetic affinity adsorbents were employed directly in a fermenter during Bacillus licheniformis cultivation to remove trace amounts of unwanted proteases. The constructed magnetic adsorbents had excellent, highly specific binding characteristics in the fermentation broth (K(d) = 1.94 micromolar; Q(max) = 222.8 mg/g), which obeyed the Langmuir isotherm and had rapid binding kinetics (equilibrium in
Original languageEnglish
JournalBiotechnology Journal
Volume7
Issue number7
Pages (from-to)909-918
ISSN1860-6768
Publication statusPublished - 2012

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