Use of ¹⁹F Differential Labelling for the Simultaneous Detection and Monitoring of Three Individual Proteins in a Serum Environment

John M. Edwards, Pernille Harris, Jens T. Bukrinski, Alexander P. Golovanov*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

Protein behavior in complex mixtures, such as biological fluids, is often modeled by simplified solution buffer systems. Here we have used the recently described differential ¹⁹F labelling approach (with NMR detection) to monitor and compare the solution behaviour of three proteins at once: human serum albumin (HSA), transferrin (TrF) and immunoglobulin G (IgG), both in serum and in buffer. We demonstrate that monitoring three proteins simultaneously and independently in biological fluid is possible, and that the presence of other endogenous components greatly changes the association characteristics of these proteins. For example, in the simplified model buffer system all three proteins diffuse at a similar rate, while in serum HSA diffuses around 3 times faster than TrF, and 4 times faster than IgG. This ¹⁹F NMR approach opens avenues to characterise the behaviour of complex multi‐protein systems in their native environment, e.g., in biological fluids.
Original languageEnglish
JournalChemPlusChem
Volume84
Issue number5
Pages (from-to)443-446
Number of pages4
ISSN2192-6506
DOIs
Publication statusPublished - 2019

Keywords

  • Diffusion
  • 19F NMR Spectroscopy
  • Multiprotein systems
  • Protein-protein interactions
  • Serum analysis

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