Use of extremophilic bacteria for second generation bioethanol production

Ana Faria Tomás, Dimitar Borisov Karakashev, Irini Angelidaki

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

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Abstract

The pursuit of ways to obtain viable alternatives to fossil fuels has been one of the main subjects in microbial biotechnology research in the last decade. Of all the possible fuel candidates, bioethanol is one of the most relevant, especially when considered for the transport sector. Its production from food crops, such as corn (starch) or sugar cane (sucrose) is already an established process, with the USA and Brazil supplying 86% of the market. The major challenge remains in the use of different waste sources – agricultural, forestry, animal and household waste - as a feedstock. The recalcitrance of these materials and their diverse sugar composition make the industrial yeast strains currently used unsuitable for a second generation bioethanol production process.
One of the alternative strategies is the use of extreme thermophilic microorganisms. Currently, selected members from the genera Clostridium, Thermoanaerobacter, Geobacillus and Thermoanaerobacterium are among the best candidates. A new strain of Thermoanaerobacter, closely related to T. italicus and T. mathranii, has achieved 0.43 gethanol/gxylose, which is 83% of the theoretical yield of ethanol based on xylose and the highest value for a wild type strain reported so far. However, productivity and titer values comparable to a first generation process are yet to be achieved. Metabolic engineering to redirect the metabolism from mixed-product fermentation to ethanol production is one of the solutions proposed to improve the performance of extreme thermophilic bacteria.
Original languageEnglish
Publication date2011
Number of pages1
Publication statusPublished - 2011
Event2011 Symposium The Danish Microbiological Society - Copenhagen, Denmark
Duration: 7 Nov 2011 → …

Conference

Conference2011 Symposium The Danish Microbiological Society
CountryDenmark
CityCopenhagen
Period07/11/2011 → …

Bibliographical note

Oral presentation

Cite this

Tomás, A. F., Karakashev, D. B., & Angelidaki, I. (2011). Use of extremophilic bacteria for second generation bioethanol production. Abstract from 2011 Symposium The Danish Microbiological Society, Copenhagen, Denmark.
Tomás, Ana Faria ; Karakashev, Dimitar Borisov ; Angelidaki, Irini. / Use of extremophilic bacteria for second generation bioethanol production. Abstract from 2011 Symposium The Danish Microbiological Society, Copenhagen, Denmark.1 p.
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Tomás, AF, Karakashev, DB & Angelidaki, I 2011, 'Use of extremophilic bacteria for second generation bioethanol production', 2011 Symposium The Danish Microbiological Society, Copenhagen, Denmark, 07/11/2011.

Use of extremophilic bacteria for second generation bioethanol production. / Tomás, Ana Faria ; Karakashev, Dimitar Borisov; Angelidaki, Irini.

2011. Abstract from 2011 Symposium The Danish Microbiological Society, Copenhagen, Denmark.

Research output: Contribution to conferenceConference abstract for conferenceResearchpeer-review

TY - ABST

T1 - Use of extremophilic bacteria for second generation bioethanol production

AU - Tomás, Ana Faria

AU - Karakashev, Dimitar Borisov

AU - Angelidaki, Irini

N1 - Oral presentation

PY - 2011

Y1 - 2011

N2 - The pursuit of ways to obtain viable alternatives to fossil fuels has been one of the main subjects in microbial biotechnology research in the last decade. Of all the possible fuel candidates, bioethanol is one of the most relevant, especially when considered for the transport sector. Its production from food crops, such as corn (starch) or sugar cane (sucrose) is already an established process, with the USA and Brazil supplying 86% of the market. The major challenge remains in the use of different waste sources – agricultural, forestry, animal and household waste - as a feedstock. The recalcitrance of these materials and their diverse sugar composition make the industrial yeast strains currently used unsuitable for a second generation bioethanol production process. One of the alternative strategies is the use of extreme thermophilic microorganisms. Currently, selected members from the genera Clostridium, Thermoanaerobacter, Geobacillus and Thermoanaerobacterium are among the best candidates. A new strain of Thermoanaerobacter, closely related to T. italicus and T. mathranii, has achieved 0.43 gethanol/gxylose, which is 83% of the theoretical yield of ethanol based on xylose and the highest value for a wild type strain reported so far. However, productivity and titer values comparable to a first generation process are yet to be achieved. Metabolic engineering to redirect the metabolism from mixed-product fermentation to ethanol production is one of the solutions proposed to improve the performance of extreme thermophilic bacteria.

AB - The pursuit of ways to obtain viable alternatives to fossil fuels has been one of the main subjects in microbial biotechnology research in the last decade. Of all the possible fuel candidates, bioethanol is one of the most relevant, especially when considered for the transport sector. Its production from food crops, such as corn (starch) or sugar cane (sucrose) is already an established process, with the USA and Brazil supplying 86% of the market. The major challenge remains in the use of different waste sources – agricultural, forestry, animal and household waste - as a feedstock. The recalcitrance of these materials and their diverse sugar composition make the industrial yeast strains currently used unsuitable for a second generation bioethanol production process. One of the alternative strategies is the use of extreme thermophilic microorganisms. Currently, selected members from the genera Clostridium, Thermoanaerobacter, Geobacillus and Thermoanaerobacterium are among the best candidates. A new strain of Thermoanaerobacter, closely related to T. italicus and T. mathranii, has achieved 0.43 gethanol/gxylose, which is 83% of the theoretical yield of ethanol based on xylose and the highest value for a wild type strain reported so far. However, productivity and titer values comparable to a first generation process are yet to be achieved. Metabolic engineering to redirect the metabolism from mixed-product fermentation to ethanol production is one of the solutions proposed to improve the performance of extreme thermophilic bacteria.

M3 - Conference abstract for conference

ER -

Tomás AF, Karakashev DB, Angelidaki I. Use of extremophilic bacteria for second generation bioethanol production. 2011. Abstract from 2011 Symposium The Danish Microbiological Society, Copenhagen, Denmark.