Upregulation of TGF-beta 1 in neonates of mothers receiving Influenza A (H1N1) vaccination during pregnancy

Anne Louise Bischoff, N. Folsgaard, H. Bisgaard, M. Rasmussen, Susanne Brix Pedersen

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

    Abstract

    Background: Influenza vaccination of pregnant women is generally considered safe,but the effects on the immune system of the unborn child are unknown.Objectives: Our primary objective was to explore differences in cytokine and chemokine levels in nasal mucosal lining fluid in neonates of mothers vaccinated during or after pregnancy. Method: IFN-c, IL-1b, IL-2, -4, -5, -10, - 12p70, -13, -17, TNF-a, IL-8, eotaxin-1,eotaxin-3, IP-10, MCP-1, MCP-4, MDC, MIP-1b, TGF-b1 and TARC were quantified in nasal mucosal lining fluid in neonates of mothers receiving Influenza A (H1N1v) vaccine during (n = 52) or after pregnancy (n = 118) in our unselected Copenhagen Prospective Study on Asthma in Childhood 2010 birth-cohort. Result: Neonates of mothers vaccinated during pregnancy showed a significant up-regulation of the immune-regulatory TGF-b1 (P = 0.0004), significant down regulation (P < 0.05) of TARC, IL-5, )8, )10, )12p70, eotaxin-1, MDC, IFN-c and non-significant down regulation of nearly all other mediators except for MCP-4,IL-17, eotaxin-3 compared to neonates of mothers vaccinated after pregnancy.Results are adjusted for season; airway colonization S. pneumoniae, H. influenzae, M.catarrhalis, and S. aureus; older siblings; furred animals in home; smoking during 3rd trimester; and mothers’ atopic disease. Conclusion: These findings suggest that Influenza A (H1N1) vaccination during pregnancy affects the mucosal immune competence of the unborn child. The up-regulation of TGF-b1 and down-regulation of nearly all essential contributors to protective immunity reflect an imprinting suggestive of immune inhibition that may affect the neonates’ ability to combat respiratory tract infections.
    Original languageEnglish
    JournalAllergy: European Journal of Allergy and Clinical Immunology
    Volume67
    Issue numbers96
    Pages (from-to)475
    ISSN0105-4538
    DOIs
    Publication statusPublished - 2012
    Event31 Congress of the European Academy of Allergy and Clinical Immunology - Geneva, Switzerland
    Duration: 16 Jun 201220 Dec 2012

    Conference

    Conference31 Congress of the European Academy of Allergy and Clinical Immunology
    CountrySwitzerland
    CityGeneva
    Period16/06/201220/12/2012

    Bibliographical note

    Poster session 58: Pediatric allergy, asthma and infection, nr. 1256, side 24

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