Universal Behavior of Membranes with Sterols

Jonas Rosager Henriksen, Amy C. Rowat, E. Brief, Y. W. Hsueh, J. L. Thewalt, M. J. Zuckermann, John H. Ipsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Lanosterol is the biosynthetic precursor of cholesterol and ergosterol, sterols that predominate in the membranes of mammals and lower eukaryotes, respectively. These three sterols are structurally quite similar, yet their relative effects on membranes have been shown to differ. Here we study the effects of cholesterol, lanosterol, and ergosterol on 1-palmitoyl-2- oleoyl-sn-glycero-3-phosphatidylcholine lipid bilayers at room temperature. Micropipette aspiration is used to determine membrane material properties (area compressibility modulus), and information about lipid chain order (first moments) is obtained from deuterium nuclear magnetic resonance. We compare these results, along with data for membrane-bending rigidity, to explore the relationship between membrane hydrophobic thickness and elastic properties. Together, such diverse approaches demonstrate that membrane properties are affected to different degrees by these structurally distinct sterols, yet nonetheless exhibit universal behavior.
    Original languageEnglish
    JournalBiophysical Journal
    Volume90
    Pages (from-to)1639-1649
    ISSN0006-3495
    DOIs
    Publication statusPublished - 2006

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