Universal Behavior of Membranes with Sterols

Jonas Rosager Henriksen; Amy C. Rowat; E. Brief; Y. W. Hsueh; J. L. Thewalt; M. J. Zuckermann; John H. Ipsen

doi:10.1529/biophysj.105.067652

Universal Behavior of Membranes with Sterols

Jonas Rosager Henriksen, Amy C. Rowat, E. Brief, Y. W. Hsueh, J. L. Thewalt, M. J. Zuckermann, John H. Ipsen

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Lanosterol is the biosynthetic precursor of cholesterol and ergosterol, sterols that predominate in the membranes of mammals and lower eukaryotes, respectively. These three sterols are structurally quite similar, yet their relative effects on membranes have been shown to differ. Here we study the effects of cholesterol, lanosterol, and ergosterol on 1-palmitoyl-2- oleoyl-sn-glycero-3-phosphatidylcholine lipid bilayers at room temperature. Micropipette aspiration is used to determine membrane material properties (area compressibility modulus), and information about lipid chain order (first moments) is obtained from deuterium nuclear magnetic resonance. We compare these results, along with data for membrane-bending rigidity, to explore the relationship between membrane hydrophobic thickness and elastic properties. Together, such diverse approaches demonstrate that membrane properties are affected to different degrees by these structurally distinct sterols, yet nonetheless exhibit universal behavior.

Original language	English
Journal	Biophysical Journal
Volume	90
Pages (from-to)	1639-1649
ISSN	0006-3495
DOIs	https://doi.org/10.1529/biophysj.105.067652
Publication status	Published - 2006

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title = "Universal Behavior of Membranes with Sterols",

abstract = "Lanosterol is the biosynthetic precursor of cholesterol and ergosterol, sterols that predominate in the membranes of mammals and lower eukaryotes, respectively. These three sterols are structurally quite similar, yet their relative effects on membranes have been shown to differ. Here we study the effects of cholesterol, lanosterol, and ergosterol on 1-palmitoyl-2- oleoyl-sn-glycero-3-phosphatidylcholine lipid bilayers at room temperature. Micropipette aspiration is used to determine membrane material properties (area compressibility modulus), and information about lipid chain order (first moments) is obtained from deuterium nuclear magnetic resonance. We compare these results, along with data for membrane-bending rigidity, to explore the relationship between membrane hydrophobic thickness and elastic properties. Together, such diverse approaches demonstrate that membrane properties are affected to different degrees by these structurally distinct sterols, yet nonetheless exhibit universal behavior.",

author = "Henriksen, {Jonas Rosager} and Rowat, {Amy C.} and E. Brief and Hsueh, {Y. W.} and Thewalt, {J. L.} and Zuckermann, {M. J.} and Ipsen, {John H.}",

year = "2006",

doi = "10.1529/biophysj.105.067652",

language = "English",

volume = "90",

pages = "1639--1649",

journal = "Biophysical Journal",

issn = "0006-3495",

publisher = "Cell Press",

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TY - JOUR

T1 - Universal Behavior of Membranes with Sterols

AU - Henriksen, Jonas Rosager

AU - Rowat, Amy C.

AU - Brief, E.

AU - Hsueh, Y. W.

AU - Thewalt, J. L.

AU - Zuckermann, M. J.

AU - Ipsen, John H.

PY - 2006

Y1 - 2006

N2 - Lanosterol is the biosynthetic precursor of cholesterol and ergosterol, sterols that predominate in the membranes of mammals and lower eukaryotes, respectively. These three sterols are structurally quite similar, yet their relative effects on membranes have been shown to differ. Here we study the effects of cholesterol, lanosterol, and ergosterol on 1-palmitoyl-2- oleoyl-sn-glycero-3-phosphatidylcholine lipid bilayers at room temperature. Micropipette aspiration is used to determine membrane material properties (area compressibility modulus), and information about lipid chain order (first moments) is obtained from deuterium nuclear magnetic resonance. We compare these results, along with data for membrane-bending rigidity, to explore the relationship between membrane hydrophobic thickness and elastic properties. Together, such diverse approaches demonstrate that membrane properties are affected to different degrees by these structurally distinct sterols, yet nonetheless exhibit universal behavior.

AB - Lanosterol is the biosynthetic precursor of cholesterol and ergosterol, sterols that predominate in the membranes of mammals and lower eukaryotes, respectively. These three sterols are structurally quite similar, yet their relative effects on membranes have been shown to differ. Here we study the effects of cholesterol, lanosterol, and ergosterol on 1-palmitoyl-2- oleoyl-sn-glycero-3-phosphatidylcholine lipid bilayers at room temperature. Micropipette aspiration is used to determine membrane material properties (area compressibility modulus), and information about lipid chain order (first moments) is obtained from deuterium nuclear magnetic resonance. We compare these results, along with data for membrane-bending rigidity, to explore the relationship between membrane hydrophobic thickness and elastic properties. Together, such diverse approaches demonstrate that membrane properties are affected to different degrees by these structurally distinct sterols, yet nonetheless exhibit universal behavior.

U2 - 10.1529/biophysj.105.067652

DO - 10.1529/biophysj.105.067652

M3 - Journal article

VL - 90

SP - 1639

EP - 1649

JO - Biophysical Journal

JF - Biophysical Journal

SN - 0006-3495

ER -