Understanding the 3-hydroxypropionic acid tolerance mechanism in Saccharomyces cerevisiae

Kanchana Rueksomtawin Kildegaard; Agnieszka Juncker; Bjorn Hallstrom; Niels Bjerg Jensen; Jerome Maury; Jen Nielsen; Jochen Förster; Irina Borodina

Understanding the 3-hydroxypropionic acid tolerance mechanism in Saccharomyces cerevisiae

Kanchana Rueksomtawin Kildegaard
, Agnieszka Juncker
, Bjorn Hallstrom
, Niels Bjerg Jensen
, Jerome Maury
, Jen Nielsen
, Jochen Förster
, Irina Borodina

Novo Nordisk Foundation Center for Biosustainability

Research output: Contribution to journal › Conference abstract in journal › Research › peer-review

Abstract

3-Hydroxypropionic acid (3HP) is an important platform chemical that can be converted into other valuable chemicals such as acrylic acid and its derivatives that are used in baby diap ers, various plastics, and paints. With the oil and gas resources becoming limiting, biotechnolo gy offers a sustainable alternative for production of acrylic acid from renewable feedstocks. We are establishing
Saccharomyces cerevisiae as an alternative host for 3HP production. However, 3HP also inhibits yeast grow th at level well below what is desired for commercial applications. Therefore, we are aiming to improve 3HP tolerance in S. cerevisiae by applying adaptive evolution approach. We have generated yeast strains with sign ificantly improved capacity for tolerating 3HP when compared to the wild-type. We will present physiolo gical characterization, genome re-sequencing, and transcriptome analysis of the evolved strains. Conseq uently, mechanism underlying 3HP tolerance will be investigated.

Original language	English
Journal	Yeast
Volume	30
Issue number	S1
ISSN	0749-503X
Publication status	Published - 2013
Event	26th International Conference on Yeast Genetics and Molecular Biology - Frankfurt am Main, Germany Duration: 29 Aug 2013 → 3 Sept 2013 Conference number: 26

Conference

Conference	26th International Conference on Yeast Genetics and Molecular Biology
Number	26
Country/Territory	Germany
City	Frankfurt am Main
Period	29/08/2013 → 03/09/2013

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 7 Affordable and Clean Energy

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title = "Understanding the 3-hydroxypropionic acid tolerance mechanism in Saccharomyces cerevisiae",

abstract = "3-Hydroxypropionic acid (3HP) is an important platform chemical that can be converted into other valuable chemicals such as acrylic acid and its derivatives that are used in baby diap ers, various plastics, and paints. With the oil and gas resources becoming limiting, biotechnolo gy offers a sustainable alternative for production of acrylic acid from renewable feedstocks. We are establishing Saccharomyces cerevisiae as an alternative host for 3HP production. However, 3HP also inhibits yeast grow th at level well below what is desired for commercial applications. Therefore, we are aiming to improve 3HP tolerance in S. cerevisiae by applying adaptive evolution approach. We have generated yeast strains with sign ificantly improved capacity for tolerating 3HP when compared to the wild-type. We will present physiolo gical characterization, genome re-sequencing, and transcriptome analysis of the evolved strains. Conseq uently, mechanism underlying 3HP tolerance will be investigated. ",

author = "Kildegaard, \{Kanchana Rueksomtawin\} and Agnieszka Juncker and Bjorn Hallstrom and Jensen, \{Niels Bjerg\} and Jerome Maury and Jen Nielsen and Jochen F{\"o}rster and Irina Borodina",

year = "2013",

language = "English",

volume = "30",

journal = "Yeast",

issn = "0749-503X",

publisher = "John Wiley and Sons Ltd",

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note = "26th International Conference on Yeast Genetics and Molecular Biology, ICYGMB 2013 ; Conference date: 29-08-2013 Through 03-09-2013",

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TY - ABST

T1 - Understanding the 3-hydroxypropionic acid tolerance mechanism in Saccharomyces cerevisiae

AU - Kildegaard, Kanchana Rueksomtawin

AU - Juncker, Agnieszka

AU - Hallstrom, Bjorn

AU - Jensen, Niels Bjerg

AU - Maury, Jerome

AU - Nielsen, Jen

AU - Förster, Jochen

AU - Borodina, Irina

N1 - Conference code: 26

PY - 2013

Y1 - 2013

N2 - 3-Hydroxypropionic acid (3HP) is an important platform chemical that can be converted into other valuable chemicals such as acrylic acid and its derivatives that are used in baby diap ers, various plastics, and paints. With the oil and gas resources becoming limiting, biotechnolo gy offers a sustainable alternative for production of acrylic acid from renewable feedstocks. We are establishing Saccharomyces cerevisiae as an alternative host for 3HP production. However, 3HP also inhibits yeast grow th at level well below what is desired for commercial applications. Therefore, we are aiming to improve 3HP tolerance in S. cerevisiae by applying adaptive evolution approach. We have generated yeast strains with sign ificantly improved capacity for tolerating 3HP when compared to the wild-type. We will present physiolo gical characterization, genome re-sequencing, and transcriptome analysis of the evolved strains. Conseq uently, mechanism underlying 3HP tolerance will be investigated.

AB - 3-Hydroxypropionic acid (3HP) is an important platform chemical that can be converted into other valuable chemicals such as acrylic acid and its derivatives that are used in baby diap ers, various plastics, and paints. With the oil and gas resources becoming limiting, biotechnolo gy offers a sustainable alternative for production of acrylic acid from renewable feedstocks. We are establishing Saccharomyces cerevisiae as an alternative host for 3HP production. However, 3HP also inhibits yeast grow th at level well below what is desired for commercial applications. Therefore, we are aiming to improve 3HP tolerance in S. cerevisiae by applying adaptive evolution approach. We have generated yeast strains with sign ificantly improved capacity for tolerating 3HP when compared to the wild-type. We will present physiolo gical characterization, genome re-sequencing, and transcriptome analysis of the evolved strains. Conseq uently, mechanism underlying 3HP tolerance will be investigated.

M3 - Conference abstract in journal

SN - 0749-503X

VL - 30

JO - Yeast

JF - Yeast

IS - S1

T2 - 26th International Conference on Yeast Genetics and Molecular Biology

Y2 - 29 August 2013 through 3 September 2013

ER -

Understanding the 3-hydroxypropionic acid tolerance mechanism in Saccharomyces cerevisiae

Abstract

Conference

UN SDGs

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