Uncoupler resistance in E. coli Tuv and Cuv is due to the exclusion of uncoupler by the outer membrane

Robert S. Haworth, Peter Ruhdal Jensen, Ole Michelsen, John A. Wyatt, Clive J. Brealey, R. Brian Beechey

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The uncoupler resistant bacterial strains E. coli Tuv and Cuv share the high deoxycholate sensitivity of the parent strain, Doc S. However, both Tuv and Cuv show greater resistance than Doc S to other detergents. Measurement of the periplasmic volume indicates that the outer membrane of Doc S is freely permeable to both TPP+ and hydroxymethylinulin. Tuv and Cuv are able to exclude these compounds. EDTA treatment was necessary prior to measuring membrane potentials in Tuv and Cuv. Under conditions where Δψ could be measured, uncouplers acted to dissipate Δψ with equal potency in all strains. Uncoupler resistant proline uptake in Tuv and Cuv was abolished by EDTA treatment. Transduction experiments with phage P1 showed that uncoupler resistance could be transferred from Tuv to Doc S. Such transductants were no longer sensitive to novobiocin. The gene for uncoupler resistance cotransduced with the gene pyrE (82 min). Plating efficiency experiments with P1 suggests that detergent sensitivity in Doc S arises from an rfa (81 min) mutation. This mutation is no longer present in Tuv.
    Original languageEnglish
    JournalBBA Bioenergetics
    Volume1019
    Issue number1
    Pages (from-to)67-72
    Number of pages6
    ISSN0005-2728
    Publication statusPublished - 1990

    Keywords

    • CCCPcarbonylcyanide-m-chlorophenylhydrazone
    • TTFB4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole
    • TPP+tetraphenylphosphonium cation
    • SDSsodium dodecyl sulphate

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