Uncertainties of size measurements in electron microscopy characterization of nanomaterials in foods

Agnieszka Dudkiewicz, Alistair B. A. Boxall, Qasim Chaudhry, Kristian Mølhave, Karen Tiede, Peter Hofmann, Thomas P. J. Linsinger

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    Abstract

    Electron microscopy is a recognized standard tool for nanomaterial characterization, and recommended by the European Food Safety Authority for the size measurement of nanomaterials in food. Despite this, little data have been published assessing the reliability of the method, especially for size measurement of nanomaterials characterized by a broad size distribution and/or added to food matrices. This study is a thorough investigation of the measurement uncertainty when applying electron microscopy for size measurement of engineered nanomaterials in foods. Our results show that the number of measured particles was only a minor source of measurement uncertainty for nanomaterials in food, compared to the combined influence of sampling, sample preparation prior to imaging and the image analysis. The main conclusion is that to improve the measurement reliability, care should be taken to consider replications and matrix removal prior to sample preparation.
    Original languageEnglish
    JournalFood Chemistry
    Volume176
    Pages (from-to)472-479
    Number of pages8
    ISSN0308-8146
    DOIs
    Publication statusPublished - 2015

    Cite this

    Dudkiewicz, A., Boxall, A. B. A., Chaudhry, Q., Mølhave, K., Tiede, K., Hofmann, P., & Linsinger, T. P. J. (2015). Uncertainties of size measurements in electron microscopy characterization of nanomaterials in foods. Food Chemistry, 176, 472-479. https://doi.org/10.1016/j.foodchem.2014.12.071