Ultrastructural and molecular distinctions between the porcine inner cell mass and epiblast reveal unique pluripotent cell states

V. J. Hall, Janus Valentin Jacobsen, M. A. Rasmussen, P. Hyttel

    Research output: Contribution to journalJournal articlepeer-review

    Abstract

    Characterization of the pluripotent cell populations within the porcine embryo is essential for understanding pluripotency and self-renewal regulation in the inner cell mass (ICM) and epiblast. In this study, we perform detailed ultrastructural and molecular characterization of the developing pluripotent cell population as it develops from the ICM to the late epiblast. The ultrastructural observations revealed that the outer cells of the ICM have a high nuclear:cytoplasmic ratio but are transcriptionally inactive and contain mitochondria with few cristae. In contrast, the epiblast cells have a reduced nuclear:cytoplasmic ratio, are more transcriptionally active, and contain abundant cellular organelles. This study also revealed cavitation and potential unfolding of the epiblast. As the ICM forms the epiblast, SSEA1 is lost and VIMENTIN is lost and re-expressed. The D6 blastocyst expressed high levels of STELLA, TERF1, and GDF3, and the epiblast expressed epithelial markers, MUC1 and E-CADHERIN, and the pluripotency markers, DNMT3B and CRIPTO.
    Original languageEnglish
    JournalDevelopmental Dynamics
    Volume239
    Issue number11
    Pages (from-to)2911-20
    ISSN1058-8388
    DOIs
    Publication statusPublished - 2010

    Fingerprint

    Dive into the research topics of 'Ultrastructural and molecular distinctions between the porcine inner cell mass and epiblast reveal unique pluripotent cell states'. Together they form a unique fingerprint.

    Cite this