Ultrasensitive probing of the protein resistance of PEG surfaces by secondary ion mass spectrometry

P. Kingshott, S. McArthur, H. Thissen, D.G. Castner, H.J. Griesser

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The highly sensitive surface analytical techniques X-ray photoelectron spectroscopy (XPS) and time-of-flight static secondary ion mass spectrometry (ToF-SIMS) were used to test the resistance of poly(ethylene glycol) (PEG) coatings towards adsorption of lysozyme (LYS) and fibronectin (FN). PEG coatings were prepared by grafting methoxy-terminated aldehyde-PEG (MW 5000 Da) onto two amino-functionalised surfaces with different amine group densities, generated by radio frequency glow discharge polymerisation of n-heptylamine and allylamine. Grafting was performed at the lower critical solution temperature to maximise the graft density of the PEG chains. XPS showed that the grafted density of PEG chains was slightly higher on the allylamine surface. XPS detected no adsorption of either protein on either PEG coating. ToF-SIMS analysis, on the other hand, found, in the positive ion spectra, minute but statistically significant signals assignable to amino acid fragment ions from both proteins adsorbed to the lower density PEG coating and from LYS but not FN on the higher density PEG coating. Negative ion spectra contained relatively more intense protein fragment ion signals for the lower density PEG coating but no changes assignable to adsorbed proteins on the higher density PEG coating. These results demonstrate the importance of utilising highly sensitive techniques to study protein adsorption on surfaces intended to be protein resistant, and that both positive and negative ion ToF-SIMS spectra should be acquired to probe for possible very low levels of protein adsorption. (C) 2002 Elsevier Science Ltd. All rights reserved.
    Original languageEnglish
    JournalBiomaterials
    Volume23
    Issue number24
    Pages (from-to)4775-4785
    ISSN0142-9612
    DOIs
    Publication statusPublished - 2002

    Cite this

    Kingshott, P., McArthur, S., Thissen, H., Castner, D. G., & Griesser, H. J. (2002). Ultrasensitive probing of the protein resistance of PEG surfaces by secondary ion mass spectrometry. Biomaterials, 23(24), 4775-4785. https://doi.org/10.1016/S0142-9612(02)00228-4
    Kingshott, P. ; McArthur, S. ; Thissen, H. ; Castner, D.G. ; Griesser, H.J. / Ultrasensitive probing of the protein resistance of PEG surfaces by secondary ion mass spectrometry. In: Biomaterials. 2002 ; Vol. 23, No. 24. pp. 4775-4785.
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    abstract = "The highly sensitive surface analytical techniques X-ray photoelectron spectroscopy (XPS) and time-of-flight static secondary ion mass spectrometry (ToF-SIMS) were used to test the resistance of poly(ethylene glycol) (PEG) coatings towards adsorption of lysozyme (LYS) and fibronectin (FN). PEG coatings were prepared by grafting methoxy-terminated aldehyde-PEG (MW 5000 Da) onto two amino-functionalised surfaces with different amine group densities, generated by radio frequency glow discharge polymerisation of n-heptylamine and allylamine. Grafting was performed at the lower critical solution temperature to maximise the graft density of the PEG chains. XPS showed that the grafted density of PEG chains was slightly higher on the allylamine surface. XPS detected no adsorption of either protein on either PEG coating. ToF-SIMS analysis, on the other hand, found, in the positive ion spectra, minute but statistically significant signals assignable to amino acid fragment ions from both proteins adsorbed to the lower density PEG coating and from LYS but not FN on the higher density PEG coating. Negative ion spectra contained relatively more intense protein fragment ion signals for the lower density PEG coating but no changes assignable to adsorbed proteins on the higher density PEG coating. These results demonstrate the importance of utilising highly sensitive techniques to study protein adsorption on surfaces intended to be protein resistant, and that both positive and negative ion ToF-SIMS spectra should be acquired to probe for possible very low levels of protein adsorption. (C) 2002 Elsevier Science Ltd. All rights reserved.",
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    Kingshott, P, McArthur, S, Thissen, H, Castner, DG & Griesser, HJ 2002, 'Ultrasensitive probing of the protein resistance of PEG surfaces by secondary ion mass spectrometry', Biomaterials, vol. 23, no. 24, pp. 4775-4785. https://doi.org/10.1016/S0142-9612(02)00228-4

    Ultrasensitive probing of the protein resistance of PEG surfaces by secondary ion mass spectrometry. / Kingshott, P.; McArthur, S.; Thissen, H.; Castner, D.G.; Griesser, H.J.

    In: Biomaterials, Vol. 23, No. 24, 2002, p. 4775-4785.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Ultrasensitive probing of the protein resistance of PEG surfaces by secondary ion mass spectrometry

    AU - Kingshott, P.

    AU - McArthur, S.

    AU - Thissen, H.

    AU - Castner, D.G.

    AU - Griesser, H.J.

    PY - 2002

    Y1 - 2002

    N2 - The highly sensitive surface analytical techniques X-ray photoelectron spectroscopy (XPS) and time-of-flight static secondary ion mass spectrometry (ToF-SIMS) were used to test the resistance of poly(ethylene glycol) (PEG) coatings towards adsorption of lysozyme (LYS) and fibronectin (FN). PEG coatings were prepared by grafting methoxy-terminated aldehyde-PEG (MW 5000 Da) onto two amino-functionalised surfaces with different amine group densities, generated by radio frequency glow discharge polymerisation of n-heptylamine and allylamine. Grafting was performed at the lower critical solution temperature to maximise the graft density of the PEG chains. XPS showed that the grafted density of PEG chains was slightly higher on the allylamine surface. XPS detected no adsorption of either protein on either PEG coating. ToF-SIMS analysis, on the other hand, found, in the positive ion spectra, minute but statistically significant signals assignable to amino acid fragment ions from both proteins adsorbed to the lower density PEG coating and from LYS but not FN on the higher density PEG coating. Negative ion spectra contained relatively more intense protein fragment ion signals for the lower density PEG coating but no changes assignable to adsorbed proteins on the higher density PEG coating. These results demonstrate the importance of utilising highly sensitive techniques to study protein adsorption on surfaces intended to be protein resistant, and that both positive and negative ion ToF-SIMS spectra should be acquired to probe for possible very low levels of protein adsorption. (C) 2002 Elsevier Science Ltd. All rights reserved.

    AB - The highly sensitive surface analytical techniques X-ray photoelectron spectroscopy (XPS) and time-of-flight static secondary ion mass spectrometry (ToF-SIMS) were used to test the resistance of poly(ethylene glycol) (PEG) coatings towards adsorption of lysozyme (LYS) and fibronectin (FN). PEG coatings were prepared by grafting methoxy-terminated aldehyde-PEG (MW 5000 Da) onto two amino-functionalised surfaces with different amine group densities, generated by radio frequency glow discharge polymerisation of n-heptylamine and allylamine. Grafting was performed at the lower critical solution temperature to maximise the graft density of the PEG chains. XPS showed that the grafted density of PEG chains was slightly higher on the allylamine surface. XPS detected no adsorption of either protein on either PEG coating. ToF-SIMS analysis, on the other hand, found, in the positive ion spectra, minute but statistically significant signals assignable to amino acid fragment ions from both proteins adsorbed to the lower density PEG coating and from LYS but not FN on the higher density PEG coating. Negative ion spectra contained relatively more intense protein fragment ion signals for the lower density PEG coating but no changes assignable to adsorbed proteins on the higher density PEG coating. These results demonstrate the importance of utilising highly sensitive techniques to study protein adsorption on surfaces intended to be protein resistant, and that both positive and negative ion ToF-SIMS spectra should be acquired to probe for possible very low levels of protein adsorption. (C) 2002 Elsevier Science Ltd. All rights reserved.

    KW - 7-I poly

    U2 - 10.1016/S0142-9612(02)00228-4

    DO - 10.1016/S0142-9612(02)00228-4

    M3 - Journal article

    VL - 23

    SP - 4775

    EP - 4785

    JO - Biomaterials

    JF - Biomaterials

    SN - 0142-9612

    IS - 24

    ER -