Ultrahigh-Field DCE-MRI of Angiogenesis in a Novel Angiogenesis Mouse Model

Thomas Wittenborn; Thomas Nielsen; Jens V. Nygaard; Esben Kjær Unmack Larsen; Troels Thim; Louise M. Rydtoft; Thomas Vorup-Jensen; Jørgen Kjems; Niels Chr. Nielsen; Michael R. Horsman; Erling Falk

doi:10.1002/jmri.22855

Ultrahigh-Field DCE-MRI of Angiogenesis in a Novel Angiogenesis Mouse Model

Thomas Wittenborn, Thomas Nielsen, Jens V. Nygaard, Esben Kjær Unmack Larsen, Troels Thim, Louise M. Rydtoft, Thomas Vorup-Jensen, Jørgen Kjems, Niels Chr. Nielsen, Michael R. Horsman, Erling Falk

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Purpose: To be able to screen and identify potential candidate agents for noninvasive imaging of diseases involving angiogenesis, a standardized in vivo angiogenesis model is needed. Angiogenesis is a common feature of many pathological conditions and has become an important target for diagnosis and treatment, with many noninvasive imaging agents emerging. Materials and Methods: Uniform scaffolds consisting of porous and flexible polycaprolactone were implanted subcutaneously in mice and studied after 1 to 6 weeks to describe the time course of angiogenesis. The model was characterized by histology and dynamic contrastenhanced magnetic resonance imaging (DCE-MRI). Results: Microscopic examination revealed progressive ingrowth of new vessels from the periphery, leading to a fully vascularized scaffold within 6 weeks. Blood flow through the new vessels, assessed by DCE-MRI, revealed peripheral vascularization corresponding to 12.3% (SD 6.1%) of scaffold area at week 1 and a more uniform and complete distribution of vessels corresponding to 84.1% (SD 16.2%) of scaffold area at week 4. Conclusion: In agreement with microscopic examination, noninvasive DCE-MRI visualized progressive development of new vessels in a novel and standardized murine angiogenesis model, making this model suitable for screening angiogenesis-related drugs and contrast agents.
Keyword: Scaffold,Angiogenesis,Immunohistochemistry,Dynamic contrast-enhanced magnetic resonance imaging

Original language	English
Journal	Journal of Magnetic Resonance Imaging
ISSN	1053-1807
DOIs	https://doi.org/10.1002/jmri.22855
Publication status	Published - 2011
Externally published	Yes

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@article{80a24284ec794001bf0e4b74014a1aa1,

title = "Ultrahigh-Field DCE-MRI of Angiogenesis in a Novel Angiogenesis Mouse Model",

abstract = "Purpose: To be able to screen and identify potential candidate agents for noninvasive imaging of diseases involving angiogenesis, a standardized in vivo angiogenesis model is needed. Angiogenesis is a common feature of many pathological conditions and has become an important target for diagnosis and treatment, with many noninvasive imaging agents emerging. Materials and Methods: Uniform scaffolds consisting of porous and flexible polycaprolactone were implanted subcutaneously in mice and studied after 1 to 6 weeks to describe the time course of angiogenesis. The model was characterized by histology and dynamic contrastenhanced magnetic resonance imaging (DCE-MRI). Results: Microscopic examination revealed progressive ingrowth of new vessels from the periphery, leading to a fully vascularized scaffold within 6 weeks. Blood flow through the new vessels, assessed by DCE-MRI, revealed peripheral vascularization corresponding to 12.3% (SD 6.1%) of scaffold area at week 1 and a more uniform and complete distribution of vessels corresponding to 84.1% (SD 16.2%) of scaffold area at week 4. Conclusion: In agreement with microscopic examination, noninvasive DCE-MRI visualized progressive development of new vessels in a novel and standardized murine angiogenesis model, making this model suitable for screening angiogenesis-related drugs and contrast agents. Keyword: Scaffold,Angiogenesis,Immunohistochemistry,Dynamic contrast-enhanced magnetic resonance imaging",

author = "Thomas Wittenborn and Thomas Nielsen and Nygaard, {Jens V.} and Larsen, {Esben Kj{\ae}r Unmack} and Troels Thim and Rydtoft, {Louise M.} and Thomas Vorup-Jensen and J{\o}rgen Kjems and Nielsen, {Niels Chr.} and Horsman, {Michael R.} and Erling Falk",

year = "2011",

doi = "10.1002/jmri.22855",

language = "English",

journal = "Journal of Magnetic Resonance Imaging",

issn = "1053-1807",

publisher = "JohnWiley & Sons, Inc.",

}

Ultrahigh-Field DCE-MRI of Angiogenesis in a Novel Angiogenesis Mouse Model. / Wittenborn, Thomas; Nielsen, Thomas; Nygaard, Jens V.; Larsen, Esben Kjær Unmack; Thim, Troels; Rydtoft, Louise M.; Vorup-Jensen, Thomas; Kjems, Jørgen; Nielsen, Niels Chr.; Horsman, Michael R.; Falk, Erling.

In: Journal of Magnetic Resonance Imaging, 2011.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Ultrahigh-Field DCE-MRI of Angiogenesis in a Novel Angiogenesis Mouse Model

AU - Wittenborn, Thomas

AU - Nielsen, Thomas

AU - Nygaard, Jens V.

AU - Larsen, Esben Kjær Unmack

AU - Thim, Troels

AU - Rydtoft, Louise M.

AU - Vorup-Jensen, Thomas

AU - Kjems, Jørgen

AU - Nielsen, Niels Chr.

AU - Horsman, Michael R.

AU - Falk, Erling

PY - 2011

Y1 - 2011

N2 - Purpose: To be able to screen and identify potential candidate agents for noninvasive imaging of diseases involving angiogenesis, a standardized in vivo angiogenesis model is needed. Angiogenesis is a common feature of many pathological conditions and has become an important target for diagnosis and treatment, with many noninvasive imaging agents emerging. Materials and Methods: Uniform scaffolds consisting of porous and flexible polycaprolactone were implanted subcutaneously in mice and studied after 1 to 6 weeks to describe the time course of angiogenesis. The model was characterized by histology and dynamic contrastenhanced magnetic resonance imaging (DCE-MRI). Results: Microscopic examination revealed progressive ingrowth of new vessels from the periphery, leading to a fully vascularized scaffold within 6 weeks. Blood flow through the new vessels, assessed by DCE-MRI, revealed peripheral vascularization corresponding to 12.3% (SD 6.1%) of scaffold area at week 1 and a more uniform and complete distribution of vessels corresponding to 84.1% (SD 16.2%) of scaffold area at week 4. Conclusion: In agreement with microscopic examination, noninvasive DCE-MRI visualized progressive development of new vessels in a novel and standardized murine angiogenesis model, making this model suitable for screening angiogenesis-related drugs and contrast agents. Keyword: Scaffold,Angiogenesis,Immunohistochemistry,Dynamic contrast-enhanced magnetic resonance imaging

AB - Purpose: To be able to screen and identify potential candidate agents for noninvasive imaging of diseases involving angiogenesis, a standardized in vivo angiogenesis model is needed. Angiogenesis is a common feature of many pathological conditions and has become an important target for diagnosis and treatment, with many noninvasive imaging agents emerging. Materials and Methods: Uniform scaffolds consisting of porous and flexible polycaprolactone were implanted subcutaneously in mice and studied after 1 to 6 weeks to describe the time course of angiogenesis. The model was characterized by histology and dynamic contrastenhanced magnetic resonance imaging (DCE-MRI). Results: Microscopic examination revealed progressive ingrowth of new vessels from the periphery, leading to a fully vascularized scaffold within 6 weeks. Blood flow through the new vessels, assessed by DCE-MRI, revealed peripheral vascularization corresponding to 12.3% (SD 6.1%) of scaffold area at week 1 and a more uniform and complete distribution of vessels corresponding to 84.1% (SD 16.2%) of scaffold area at week 4. Conclusion: In agreement with microscopic examination, noninvasive DCE-MRI visualized progressive development of new vessels in a novel and standardized murine angiogenesis model, making this model suitable for screening angiogenesis-related drugs and contrast agents. Keyword: Scaffold,Angiogenesis,Immunohistochemistry,Dynamic contrast-enhanced magnetic resonance imaging

U2 - 10.1002/jmri.22855

DO - 10.1002/jmri.22855

M3 - Journal article

C2 - 22031493

JO - Journal of Magnetic Resonance Imaging

JF - Journal of Magnetic Resonance Imaging

SN - 1053-1807

ER -