Purpose: To be able to screen and identify potential candidate
agents for noninvasive imaging of diseases involving
angiogenesis, a standardized in vivo angiogenesis
model is needed. Angiogenesis is a common feature of
many pathological conditions and has become an important
target for diagnosis and treatment, with many noninvasive
imaging agents emerging.
Materials and Methods: Uniform scaffolds consisting of
porous and flexible polycaprolactone were implanted subcutaneously
in mice and studied after 1 to 6 weeks to
describe the time course of angiogenesis. The model was
characterized by histology and dynamic contrastenhanced
magnetic resonance imaging (DCE-MRI).
Results: Microscopic examination revealed progressive
ingrowth of new vessels from the periphery, leading to a fully vascularized scaffold within 6 weeks. Blood flow
through the new vessels, assessed by DCE-MRI, revealed
peripheral vascularization corresponding to 12.3% (SD
6.1%) of scaffold area at week 1 and a more uniform and
complete distribution of vessels corresponding to 84.1%
(SD 16.2%) of scaffold area at week 4.
Conclusion: In agreement with microscopic examination,
noninvasive DCE-MRI visualized progressive development
of new vessels in a novel and standardized murine angiogenesis
model, making this model suitable for screening
angiogenesis-related drugs and contrast agents.
Keyword: Scaffold,Angiogenesis,Immunohistochemistry,Dynamic contrast-enhanced magnetic resonance imaging