Typing of the fish pathogen Listonella (Vibrio) anguillara by pyrolysis mass spectrometry

GP Manfio; M. Goodfellow; B. Austin; DA Austin; Karl Pedersen; J.L. Larsen; L. Verdonck; J. Swings

Typing of the fish pathogen Listonella (Vibrio) anguillara by pyrolysis mass spectrometry

GP Manfio
, M. Goodfellow
, B. Austin
, DA Austin
, Karl Pedersen
, J.L. Larsen
, L. Verdonck
, J. Swings

University of Copenhagen

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

Twenty-eight representatives of Listonella (Vibrio) anguillara serovars 01, 02 and 03 were compared by Curie-point pyrolysis mass spectrometry (PyMS). The representatives of serovars 01 and 03 formed discrete, homogeneous groups in ordination plots of the PyMS data. Strains from serovar 02 were recovered in two groups, one of which encompassed six strains including the type strain of the species and the reference strain for serovar 02, and the other included two strains which showed cross-reactions between serovars 02 and 05. The almost complete agreement found between the PyMS and the serological data suggests that pyrolysis mass spectrometry will prove to be an effective method for interstrain comparison within the species Listonella anguillara.

Original language	English
Journal	Zentralblatt fur Bakteriologie
Volume	285
Issue number	2
Pages (from-to)	245-251
Number of pages	7
ISSN	0934-8840
Publication status	Published - 1997
Externally published	Yes

Keywords

Animals
Bacterial Typing Techniques
Fishes
Mass Spectrometry
Vibrio
MICROBIOLOGY
VIROLOGY
NOSOCOMIAL INFECTION
STRAIN DIFFERENTIATION
CURIE-POINT PYROLYSIS MASS SPECTROMETRY
IDENTIFICATION METHOD
INTER-STRAIN COMPARISON
METHODOLOGY
PATHOGEN
SEROVAR-O1
SEROVAR-O2
SEROVAR-O3
SEROVAR-O5
STRAIN TYPING
Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Listonella anguillara
Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] fish Pisces
30000, Bacteriology, general and systematic
Systematics and Taxonomy

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title = "Typing of the fish pathogen Listonella (Vibrio) anguillara by pyrolysis mass spectrometry",

abstract = "Twenty-eight representatives of Listonella (Vibrio) anguillara serovars 01, 02 and 03 were compared by Curie-point pyrolysis mass spectrometry (PyMS). The representatives of serovars 01 and 03 formed discrete, homogeneous groups in ordination plots of the PyMS data. Strains from serovar 02 were recovered in two groups, one of which encompassed six strains including the type strain of the species and the reference strain for serovar 02, and the other included two strains which showed cross-reactions between serovars 02 and 05. The almost complete agreement found between the PyMS and the serological data suggests that pyrolysis mass spectrometry will prove to be an effective method for interstrain comparison within the species Listonella anguillara.",

keywords = "Animals, Bacterial Typing Techniques, Fishes, Mass Spectrometry, Vibrio, MICROBIOLOGY, VIROLOGY, NOSOCOMIAL INFECTION, STRAIN DIFFERENTIATION, CURIE-POINT PYROLYSIS MASS SPECTROMETRY, IDENTIFICATION METHOD, INTER-STRAIN COMPARISON, METHODOLOGY, PATHOGEN, SEROVAR-O1, SEROVAR-O2, SEROVAR-O3, SEROVAR-O5, STRAIN TYPING, Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Listonella anguillara, Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] fish Pisces, 30000, Bacteriology, general and systematic, Systematics and Taxonomy",

author = "GP Manfio and M. Goodfellow and B. Austin and DA Austin and Karl Pedersen and J.L. Larsen and L. Verdonck and J. Swings",

year = "1997",

language = "English",

volume = "285",

pages = "245--251",

journal = "Zentralblatt fur Bakteriologie",

issn = "0934-8840",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Typing of the fish pathogen Listonella (Vibrio) anguillara by pyrolysis mass spectrometry

AU - Manfio, GP

AU - Goodfellow, M.

AU - Austin, B.

AU - Austin, DA

AU - Pedersen, Karl

AU - Larsen, J.L.

AU - Verdonck, L.

AU - Swings, J.

PY - 1997

Y1 - 1997

N2 - Twenty-eight representatives of Listonella (Vibrio) anguillara serovars 01, 02 and 03 were compared by Curie-point pyrolysis mass spectrometry (PyMS). The representatives of serovars 01 and 03 formed discrete, homogeneous groups in ordination plots of the PyMS data. Strains from serovar 02 were recovered in two groups, one of which encompassed six strains including the type strain of the species and the reference strain for serovar 02, and the other included two strains which showed cross-reactions between serovars 02 and 05. The almost complete agreement found between the PyMS and the serological data suggests that pyrolysis mass spectrometry will prove to be an effective method for interstrain comparison within the species Listonella anguillara.

AB - Twenty-eight representatives of Listonella (Vibrio) anguillara serovars 01, 02 and 03 were compared by Curie-point pyrolysis mass spectrometry (PyMS). The representatives of serovars 01 and 03 formed discrete, homogeneous groups in ordination plots of the PyMS data. Strains from serovar 02 were recovered in two groups, one of which encompassed six strains including the type strain of the species and the reference strain for serovar 02, and the other included two strains which showed cross-reactions between serovars 02 and 05. The almost complete agreement found between the PyMS and the serological data suggests that pyrolysis mass spectrometry will prove to be an effective method for interstrain comparison within the species Listonella anguillara.

KW - Animals

KW - Bacterial Typing Techniques

KW - Fishes

KW - Mass Spectrometry

KW - Vibrio

KW - MICROBIOLOGY

KW - VIROLOGY

KW - NOSOCOMIAL INFECTION

KW - STRAIN DIFFERENTIATION

KW - CURIE-POINT PYROLYSIS MASS SPECTROMETRY

KW - IDENTIFICATION METHOD

KW - INTER-STRAIN COMPARISON

KW - METHODOLOGY

KW - PATHOGEN

KW - SEROVAR-O1

KW - SEROVAR-O2

KW - SEROVAR-O3

KW - SEROVAR-O5

KW - STRAIN TYPING

KW - Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Vibrionaceae [06704] Listonella anguillara

KW - Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Pisces [85200] fish Pisces

KW - 30000, Bacteriology, general and systematic

KW - Systematics and Taxonomy

M3 - Journal article

SN - 0934-8840

VL - 285

SP - 245

EP - 251

JO - Zentralblatt fur Bakteriologie

JF - Zentralblatt fur Bakteriologie

IS - 2

ER -

Typing of the fish pathogen Listonella (Vibrio) anguillara by pyrolysis mass spectrometry

Abstract

Keywords

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