Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison)

Charlotte Mark Salomonsen, G. E. Themudo, Lars Jelsbak, Søren Molin, N. Høiby, Anne Sofie Hammer

    Research output: Contribution to journalJournal articleResearchpeer-review

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    Abstract

    Hemorrhagic pneumonia in mink (Neovison vison) is caused by Pseudomonas aeruginosa and is an acute and fatal disease in farmed mink. Earlier work has demonstrated that some outbreaks of hemorrhagic pneumonia are caused by pathogenic strains while most outbreaks are caused by local strains. The objective of this study was to determine the genetic and geographical relationship among outbreaks of hemorrhagic pneumonia by pulsed field gel electrophoresis typing of P. aeruginosa isolates. Furthermore, chosen isolates were typed by a commercial genotyping method based on single nucleotide polymorphisms (SNPs) and compared to a larger dataset of human and environmental origin. The bacterial isolates were obtained from diagnostic samples from 2002-2009 and contained 164 isolates from 95 outbreaks on 90 farms. Our results show that most outbreaks of hemorrhagic pneumonia in mink are caused by distinct strains of P. aeruginosa. We also identified related P. aeruginosa strains which, together with two prevalent but unrelated clones, caused one third of the outbreaks of hemorrhagic pneumonia supporting the sparse literature on this subject. None of the SNP typed strains were identified in a large dataset of human and environmental origin.
    Original languageEnglish
    JournalVeterinary Microbiology
    Volume163
    Issue number1-2
    Pages (from-to)103-109
    ISSN0378-1135
    DOIs
    Publication statusPublished - 2013

    Keywords

    • Pseudomonas aeruginosa
    • Mink
    • Hemorrhagic pneumonia
    • Pulsed-field gel electrophoresis
    • Single nucleotide polymorphisms

    Cite this

    Salomonsen, Charlotte Mark ; Themudo, G. E. ; Jelsbak, Lars ; Molin, Søren ; Høiby, N. ; Hammer , Anne Sofie . / Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison). In: Veterinary Microbiology. 2013 ; Vol. 163, No. 1-2. pp. 103-109.
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    abstract = "Hemorrhagic pneumonia in mink (Neovison vison) is caused by Pseudomonas aeruginosa and is an acute and fatal disease in farmed mink. Earlier work has demonstrated that some outbreaks of hemorrhagic pneumonia are caused by pathogenic strains while most outbreaks are caused by local strains. The objective of this study was to determine the genetic and geographical relationship among outbreaks of hemorrhagic pneumonia by pulsed field gel electrophoresis typing of P. aeruginosa isolates. Furthermore, chosen isolates were typed by a commercial genotyping method based on single nucleotide polymorphisms (SNPs) and compared to a larger dataset of human and environmental origin. The bacterial isolates were obtained from diagnostic samples from 2002-2009 and contained 164 isolates from 95 outbreaks on 90 farms. Our results show that most outbreaks of hemorrhagic pneumonia in mink are caused by distinct strains of P. aeruginosa. We also identified related P. aeruginosa strains which, together with two prevalent but unrelated clones, caused one third of the outbreaks of hemorrhagic pneumonia supporting the sparse literature on this subject. None of the SNP typed strains were identified in a large dataset of human and environmental origin.",
    keywords = "Pseudomonas aeruginosa, Mink, Hemorrhagic pneumonia, Pulsed-field gel electrophoresis, Single nucleotide polymorphisms",
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    Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison). / Salomonsen, Charlotte Mark; Themudo, G. E.; Jelsbak, Lars; Molin, Søren; Høiby, N.; Hammer , Anne Sofie .

    In: Veterinary Microbiology, Vol. 163, No. 1-2, 2013, p. 103-109.

    Research output: Contribution to journalJournal articleResearchpeer-review

    TY - JOUR

    T1 - Typing of Pseudomonas aeruginosa from hemorrhagic pneumonia in mink (Neovison vison)

    AU - Salomonsen, Charlotte Mark

    AU - Themudo, G. E.

    AU - Jelsbak, Lars

    AU - Molin, Søren

    AU - Høiby, N.

    AU - Hammer , Anne Sofie

    PY - 2013

    Y1 - 2013

    N2 - Hemorrhagic pneumonia in mink (Neovison vison) is caused by Pseudomonas aeruginosa and is an acute and fatal disease in farmed mink. Earlier work has demonstrated that some outbreaks of hemorrhagic pneumonia are caused by pathogenic strains while most outbreaks are caused by local strains. The objective of this study was to determine the genetic and geographical relationship among outbreaks of hemorrhagic pneumonia by pulsed field gel electrophoresis typing of P. aeruginosa isolates. Furthermore, chosen isolates were typed by a commercial genotyping method based on single nucleotide polymorphisms (SNPs) and compared to a larger dataset of human and environmental origin. The bacterial isolates were obtained from diagnostic samples from 2002-2009 and contained 164 isolates from 95 outbreaks on 90 farms. Our results show that most outbreaks of hemorrhagic pneumonia in mink are caused by distinct strains of P. aeruginosa. We also identified related P. aeruginosa strains which, together with two prevalent but unrelated clones, caused one third of the outbreaks of hemorrhagic pneumonia supporting the sparse literature on this subject. None of the SNP typed strains were identified in a large dataset of human and environmental origin.

    AB - Hemorrhagic pneumonia in mink (Neovison vison) is caused by Pseudomonas aeruginosa and is an acute and fatal disease in farmed mink. Earlier work has demonstrated that some outbreaks of hemorrhagic pneumonia are caused by pathogenic strains while most outbreaks are caused by local strains. The objective of this study was to determine the genetic and geographical relationship among outbreaks of hemorrhagic pneumonia by pulsed field gel electrophoresis typing of P. aeruginosa isolates. Furthermore, chosen isolates were typed by a commercial genotyping method based on single nucleotide polymorphisms (SNPs) and compared to a larger dataset of human and environmental origin. The bacterial isolates were obtained from diagnostic samples from 2002-2009 and contained 164 isolates from 95 outbreaks on 90 farms. Our results show that most outbreaks of hemorrhagic pneumonia in mink are caused by distinct strains of P. aeruginosa. We also identified related P. aeruginosa strains which, together with two prevalent but unrelated clones, caused one third of the outbreaks of hemorrhagic pneumonia supporting the sparse literature on this subject. None of the SNP typed strains were identified in a large dataset of human and environmental origin.

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    KW - Hemorrhagic pneumonia

    KW - Pulsed-field gel electrophoresis

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