Abstract
Degradation products of the C-telopeptides from type I collagen (CTC) can be measured with commercially available immunoassays (e.g., CrossLaps(TM) assays). It is well established that the urinary excretion of CTC fragments is closely correlated with the rate of bone degradation (resorption). Data obtained with a recently developed assay for CTC fragments in serum also suggest that serum CTC is a sensitive and specific index of bone resorption. Several structures of the CTC fragments have been elucidated. It has become clear that the peptide sequence measured in the CrossLaps assays can spontaneously beta-isomerize to produce unusual isoaspartyl peptides. This sign of protein aging is believed to ensure that the urinary CrossLaps ELISA and the Serum CrossLaps One Step ELISA, both specific for beta-isomerized fragments, measure degradation of relatively old bone. Conversely, the alpha-CrossLaps RIA, specific for non-isomerized CTC fragments, measures degradation of relatively young bone. Currently, the assays are being evaluated to clarify their potential clinical applications. They have also been shown to be efficient tools for monitoring antiresorptive therapy. Assessment of future risk of bone loss and fracture is being investigated with promising results. It is expected that the routine use of the CrossLaps assays will become established within the next few years.
Original language | English |
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Journal | Journal of Clinical Ligand Assay |
Volume | 21 |
Issue number | 2 |
Pages (from-to) | 118-127 |
Number of pages | 10 |
ISSN | 1081-1672 |
Publication status | Published - 1998 |
Externally published | Yes |
Keywords
- biochemical marker
- collagen type 1
- amino acid sequence
- article
- blood level
- bone atrophy
- diagnostic value
- fracture
- human
- immunoassay
- isomerism
- ossification
- osteolysis
- protein analysis
- risk assessment
- urine level
- Biochemical bone marker
- C-telopeptide
- Collagen type I
- CrossLaps
- BIOCHEMICAL
- IMMUNOLOGY
- MEDICAL
- LATE POSTMENOPAUSAL WOMEN
- CROSS-LINKS
- FOLLOW-UP
- ASPARAGINYL RESIDUES
- BIOCHEMICAL MARKERS
- IMMUNOASSAY
- TURNOVER
- OSTEOPOROSIS
- URINE
- MASS
- collagen type I
- biochemical bone marker
- Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae [86215] human
- type I collagen C-telopeptide degradation products bone resorption marker, urinary excretion
- 10060, Biochemistry studies - General
- 18001, Bones, joints, fasciae, connective and adipose tissue - General and methods
- Movement and Support
- antiresorptive therapy monitoring monitoring method
- CrossLaps assays analytical method
- Skeletal System