Abstract
Uracil phosphoribosyltransferase (UPRTase) catalyzes the key reaction in the salvage of uracil in many microorganisms. Surprisingly, two genes encoding UPRTase activity were cloned from Bacillus subtilis by complementation of an Escherichia coli mutant. The genes were sequenced, and the putative amino acid sequences were deduced. One gene showed a high level of homology to UPRTases from other organisms, whereas the other gene with a low level of homology to other UPRTases turned out to be the pyrR gene - the repressor of the pyr operon. The role of these genes in uracil metabolism was established by an analysis of the phenotypes of upp and pyrR mutants.
| Original language | English |
|---|---|
| Journal | Journal of Bacteriology |
| Volume | 177 |
| Issue number | 1 |
| Pages (from-to) | 271-274 |
| Number of pages | 4 |
| ISSN | 0021-9193 |
| DOIs | |
| Publication status | Published - 1995 |
Keywords
- Amino Acid Sequence
- Bacillus subtilis
- Bacterial Proteins
- Genes, Bacterial
- Molecular Sequence Data
- Pentosyltransferases
- Repressor Proteins
- Sequence Homology, Amino Acid
- Uracil
- 56HH86ZVCT Uracil
- EC 2.4.2.- Pentosyltransferases
- EC 2.4.2.- PyrR protein, bacteria
- EC 2.4.2.9 uracil phosphoribosyltransferase
- HOMOLOGY
- PYR OPERON REPRESSING PYRR GENE
- URACIL METABOLIC ROLE
- Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Endospore-forming Gram-Positives [07810] endospore-forming gram-positive rods and cocci Bacillus subtilis
- Facultatively Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Enterobacteriaceae [06702] Escherichia coli
- URACIL 66-22-8
- URACIL PHOSPHORIBOSYLTRANSFERASE 9030-24-4
- 10010, Comparative biochemistry
- 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
- 10064, Biochemistry studies - Proteins, peptides and amino acids
- 10506, Biophysics - Molecular properties and macromolecules
- 10802, Enzymes - General and comparative studies: coenzymes
- 10806, Enzymes - Chemical and physical
- 10808, Enzymes - Physiological studies
- 13014, Metabolism - Nucleic acids, purines and pyrimidines
- 31000, Physiology and biochemistry of bacteria
- 31500, Genetics of bacteria and viruses
- Biochemistry and Molecular Biophysics
- amino acid sequence
- molecular sequence data
- nucleotide sequence
- Z38002 EMBL
- Enzymology
- Genetics
- Metabolism
- Physiology
- BACILLUS subtilis