Tutorial: multiphoton microscopy to advance neuroscience research

Iván Coto Hernández; Jenny Yau; Lars Rishøj; Nanke Cui; Steven Minderler; Nate Jowett

doi:10.1088/2050-6120/acba66

Tutorial: multiphoton microscopy to advance neuroscience research

Iván Coto Hernández, Jenny Yau, Lars Rishøj, Nanke Cui, Steven Minderler, Nate Jowett

Massachusetts General Hospital/Harvard Medical School

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Abstract

Multiphoton microscopy (MPM) employs ultrafast infrared lasers for high-resolution deep three-dimensional imaging of live biological samples. The goal of this tutorial is to provide a practical guide to MPM imaging for novice microscopy developers and life-science users. Principles of MPM, microscope setup, and labeling strategies are discussed. Use of MPM to achieve unprecedented imaging depth of whole mounted explants and intravital imaging via implantable glass windows of the mammalian nervous system is demonstrated.

Original language	English
Article number	013001
Journal	Methods and Applications in Fluorescence
Volume	11
Issue number	1
Number of pages	44
ISSN	2050-6120
DOIs	https://doi.org/10.1088/2050-6120/acba66
Publication status	Published - 2023

Keywords

Laser
Multiphoton microscopy
Neuroscience
Peripheral nerve block

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AU - Jowett, Nate

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Tutorial: multiphoton microscopy to advance neuroscience research

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