Tunable promoters in systems biology.

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    The construction of synthetic promoter libraries has represented a major breakthrough in systems biology, enabling the subtle tuning of enzyme activities. A number of tools are now available that allow the modulation of gene expression and the detection of changes in expression patterns. But, how does one choose the correct promoter and what are the appropriate methods for reading promoter strength? Furthermore, how fine should the tuning of gene expression be for some specific applications and how can the simultaneous and individual tuning of multiple genes be achieved? Some recent studies have helped us to find answers to many of these questions.
    Original languageEnglish
    JournalCurrent Opinion in Biotechnology
    Issue number3
    Pages (from-to)329-335
    Publication statusPublished - 2005


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