Tunable Ampere phase plate for low dose imaging of biomolecular complexes

Amir H. Tavabi; Marco Beleggia; Vadim Migunov; Alexey Savenko; Ozan Öktem; Rafal E. Dunin-Borkowski; Giulio Pozzi

doi:10.1038/s41598-018-23100-3

Tunable Ampere phase plate for low dose imaging of biomolecular complexes

Amir H. Tavabi^*
, Marco Beleggia
, Vadim Migunov
, Alexey Savenko
, Ozan Öktem
, Rafal E. Dunin-Borkowski
, Giulio Pozzi

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › Research › peer-review

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Abstract

A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere's law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules.

Original language	English
Article number	5592
Journal	Scientific Reports
Volume	8
Issue number	1
Number of pages	5
ISSN	2045-2322
DOIs	https://doi.org/10.1038/s41598-018-23100-3
Publication status	Published - 2018

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abstract = "A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere's law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules.",

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AU - Tavabi, Amir H.

AU - Beleggia, Marco

AU - Migunov, Vadim

AU - Savenko, Alexey

AU - Öktem, Ozan

AU - Dunin-Borkowski, Rafal E.

AU - Pozzi, Giulio

PY - 2018

Y1 - 2018

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AB - A novel device that can be used as a tunable support-free phase plate for transmission electron microscopy of weakly scattering specimens is described. The device relies on the generation of a controlled phase shift by the magnetic field of a segment of current-carrying wire that is oriented parallel or antiparallel to the electron beam. The validity of the concept is established using both experimental electron holographic measurements and a theoretical model based on Ampere's law. Computer simulations are used to illustrate the resulting contrast enhancement for studies of biological cells and macromolecules.

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Tunable Ampere phase plate for low dose imaging of biomolecular complexes

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