Trophic interactions, toxicokinetics, and detoxification processes in a domoic acid-producing diatom and two copepod species

Sara Hardardottir*, Ditte Marie Hjort, Sylke Wohlrab, Bernd Krock, Uwe John, Torkel Gissel Nielsen, Nina Lundholm

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

In costal ecosystems, copepods coexist with toxin-producing phytoplankton. The presence of copepods can amplify the phytoplankton toxin production and thereby increase the overall toxicity of a bloom. Copepods are not always affected by the toxins and can vector the toxins to higher trophic levels. To investigate the interactions between toxin producers and their grazers, we determined the kinetics of grazer-induced increases in toxin production and the subsequent toxin reduction in a domoic acid (DA)-producing diatom, Pseudo-nitzschia seriata. The cellular DA level of the diatom was within the range of in situ measurements. Ten days after removal of the copepods, 28% 8% of the cellular DA still remained in the cells. Simultaneously, we monitored the
toxicokinetics of DA in two grazers; Calanus finmarchicus and Calanus glacialis. After 144 h of grazing on the toxic diet, the copepods accumulated and retained high concentrations of DA. Nine hours after exposure to the toxic diet was terminated, the copepods had depurated 70% ± 10% of the DA. The depuration lasted 4 ± 2 d and was independent of Calanus species and treatment. We explored the possible physiological responses in copepods after feeding on a purely toxic diet from gene expression profiles of C. finmarchicus. Expression of genes regulating several major metabolic and cellular processes was reduced in copepods feeding on DA-containing diatoms, and we hypothesize that this is because of exposure to DA
Original languageEnglish
JournalLimnology and Oceanography
Volume64
Issue number3
Pages (from-to)833-848
Number of pages16
ISSN0024-3590
DOIs
Publication statusPublished - 2019

Cite this

Hardardottir, Sara ; Hjort, Ditte Marie ; Wohlrab, Sylke ; Krock, Bernd ; John, Uwe ; Nielsen, Torkel Gissel ; Lundholm, Nina. / Trophic interactions, toxicokinetics, and detoxification processes in a domoic acid-producing diatom and two copepod species. In: Limnology and Oceanography. 2019 ; Vol. 64, No. 3. pp. 833-848.
@article{f9b4d62bd2834be3bee8b0e76d1ee804,
title = "Trophic interactions, toxicokinetics, and detoxification processes in a domoic acid-producing diatom and two copepod species",
abstract = "In costal ecosystems, copepods coexist with toxin-producing phytoplankton. The presence of copepods can amplify the phytoplankton toxin production and thereby increase the overall toxicity of a bloom. Copepods are not always affected by the toxins and can vector the toxins to higher trophic levels. To investigate the interactions between toxin producers and their grazers, we determined the kinetics of grazer-induced increases in toxin production and the subsequent toxin reduction in a domoic acid (DA)-producing diatom, Pseudo-nitzschia seriata. The cellular DA level of the diatom was within the range of in situ measurements. Ten days after removal of the copepods, 28{\%} 8{\%} of the cellular DA still remained in the cells. Simultaneously, we monitored thetoxicokinetics of DA in two grazers; Calanus finmarchicus and Calanus glacialis. After 144 h of grazing on the toxic diet, the copepods accumulated and retained high concentrations of DA. Nine hours after exposure to the toxic diet was terminated, the copepods had depurated 70{\%} ± 10{\%} of the DA. The depuration lasted 4 ± 2 d and was independent of Calanus species and treatment. We explored the possible physiological responses in copepods after feeding on a purely toxic diet from gene expression profiles of C. finmarchicus. Expression of genes regulating several major metabolic and cellular processes was reduced in copepods feeding on DA-containing diatoms, and we hypothesize that this is because of exposure to DA",
author = "Sara Hardardottir and Hjort, {Ditte Marie} and Sylke Wohlrab and Bernd Krock and Uwe John and Nielsen, {Torkel Gissel} and Nina Lundholm",
year = "2019",
doi = "10.1002/lno.11078",
language = "English",
volume = "64",
pages = "833--848",
journal = "Limnology and Oceanography",
issn = "0024-3590",
publisher = "JohnWiley & Sons, Inc.",
number = "3",

}

Trophic interactions, toxicokinetics, and detoxification processes in a domoic acid-producing diatom and two copepod species. / Hardardottir, Sara; Hjort, Ditte Marie; Wohlrab, Sylke; Krock, Bernd; John, Uwe; Nielsen, Torkel Gissel; Lundholm, Nina.

In: Limnology and Oceanography, Vol. 64, No. 3, 2019, p. 833-848.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Trophic interactions, toxicokinetics, and detoxification processes in a domoic acid-producing diatom and two copepod species

AU - Hardardottir, Sara

AU - Hjort, Ditte Marie

AU - Wohlrab, Sylke

AU - Krock, Bernd

AU - John, Uwe

AU - Nielsen, Torkel Gissel

AU - Lundholm, Nina

PY - 2019

Y1 - 2019

N2 - In costal ecosystems, copepods coexist with toxin-producing phytoplankton. The presence of copepods can amplify the phytoplankton toxin production and thereby increase the overall toxicity of a bloom. Copepods are not always affected by the toxins and can vector the toxins to higher trophic levels. To investigate the interactions between toxin producers and their grazers, we determined the kinetics of grazer-induced increases in toxin production and the subsequent toxin reduction in a domoic acid (DA)-producing diatom, Pseudo-nitzschia seriata. The cellular DA level of the diatom was within the range of in situ measurements. Ten days after removal of the copepods, 28% 8% of the cellular DA still remained in the cells. Simultaneously, we monitored thetoxicokinetics of DA in two grazers; Calanus finmarchicus and Calanus glacialis. After 144 h of grazing on the toxic diet, the copepods accumulated and retained high concentrations of DA. Nine hours after exposure to the toxic diet was terminated, the copepods had depurated 70% ± 10% of the DA. The depuration lasted 4 ± 2 d and was independent of Calanus species and treatment. We explored the possible physiological responses in copepods after feeding on a purely toxic diet from gene expression profiles of C. finmarchicus. Expression of genes regulating several major metabolic and cellular processes was reduced in copepods feeding on DA-containing diatoms, and we hypothesize that this is because of exposure to DA

AB - In costal ecosystems, copepods coexist with toxin-producing phytoplankton. The presence of copepods can amplify the phytoplankton toxin production and thereby increase the overall toxicity of a bloom. Copepods are not always affected by the toxins and can vector the toxins to higher trophic levels. To investigate the interactions between toxin producers and their grazers, we determined the kinetics of grazer-induced increases in toxin production and the subsequent toxin reduction in a domoic acid (DA)-producing diatom, Pseudo-nitzschia seriata. The cellular DA level of the diatom was within the range of in situ measurements. Ten days after removal of the copepods, 28% 8% of the cellular DA still remained in the cells. Simultaneously, we monitored thetoxicokinetics of DA in two grazers; Calanus finmarchicus and Calanus glacialis. After 144 h of grazing on the toxic diet, the copepods accumulated and retained high concentrations of DA. Nine hours after exposure to the toxic diet was terminated, the copepods had depurated 70% ± 10% of the DA. The depuration lasted 4 ± 2 d and was independent of Calanus species and treatment. We explored the possible physiological responses in copepods after feeding on a purely toxic diet from gene expression profiles of C. finmarchicus. Expression of genes regulating several major metabolic and cellular processes was reduced in copepods feeding on DA-containing diatoms, and we hypothesize that this is because of exposure to DA

U2 - 10.1002/lno.11078

DO - 10.1002/lno.11078

M3 - Journal article

VL - 64

SP - 833

EP - 848

JO - Limnology and Oceanography

JF - Limnology and Oceanography

SN - 0024-3590

IS - 3

ER -